GAPDH 抗体 (N-Term)
(2 references)Quick Overview for GAPDH 抗体 (N-Term) (ABIN966181)
抗原
See all GAPDH 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- No cross reactivity with other proteins.
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产品特性
- Rabbit IgG polyclonal antibody for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminal of human GAPDH , identical to the related mouse and rat sequences.< br/>Immunogen was affinity purified.
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亚型
- IgG
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibodyVerified GAPDH 适用: 人, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibodyVerified GAPDH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibodyGAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibodyGAPDH 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 斑马鱼 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibodyGAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibodyGAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibodyGAPDH 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal D1 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibodyGAPDH 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibodyGAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Sodium azide, Thimerosal (Merthiolate)
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.
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: "GAPDH and autophagy preserve survival after apoptotic cytochrome c release in the absence of caspase activation." in: Cell, Vol. 129, Issue 5, pp. 983-97, (2007) (PubMed).
: "Members of the human glyceraldehyde-3-phosphate dehydrogenase-related gene family map to dispersed chromosomal locations." in: Genomics, Vol. 5, Issue 2, pp. 209-14, (1989) (PubMed).
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: "GAPDH and autophagy preserve survival after apoptotic cytochrome c release in the absence of caspase activation." in: Cell, Vol. 129, Issue 5, pp. 983-97, (2007) (PubMed).
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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别名
- Glyceraldehyde-3-phosphate dehydrogenase(GAPDH)
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背景
- Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme is thought to be a tetramer of identical chains. Several highly homologous glyceraldehyde-3-phosphate dehydrogenase (GAPD)-related sequences have been identified previously in human DNA by Southern blot analysis. Protein studies have identified only a single expressed locus for this major glycolytic enzyme, and this maps to chromosome 12p13. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a critical regulator of CICD, it mediates an elevation in glycolysis and enhanced autophagy that cooperate to protect cells from CICD.
抗原
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