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GAPDH 抗体 (N-Term)

Cited in 2+ publications. This anti-GAPDH antibody is a 兔 多克隆 antibody detecting GAPDH in WB, ICC, IHC (p) 和 IHC (f). Suitable for 人.
产品编号 ABIN966181
发货至: 中国
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for GAPDH 抗体 (N-Term) (ABIN966181)

抗原

See all GAPDH 抗体
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

适用

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宿主

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克隆类型

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多克隆

标记

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This GAPDH antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
  • 抗原表位

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    N-Term

    交叉反应

    小鼠, 大鼠

    交叉反应 (详细)

    No cross reactivity with other proteins.

    产品特性

    Rabbit IgG polyclonal antibody for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.

    免疫原

    A synthetic peptide corresponding to a sequence at the N-terminal of human GAPDH , identical to the related mouse and rat sequences.< br/>Immunogen was affinity purified.

    亚型

    IgG
  • 限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

    储存液

    Sodium azide, Thimerosal (Merthiolate)

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C

    储存方法

    At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.
  • Colell, Ricci, Tait, Milasta, Maurer, Bouchier-Hayes, Fitzgerald, Guio-Carrion, Waterhouse, Li, Mari, Barbry, Newmeyer, Beere, Green: "GAPDH and autophagy preserve survival after apoptotic cytochrome c release in the absence of caspase activation." in: Cell, Vol. 129, Issue 5, pp. 983-97, (2007) (PubMed).

    Benham, Povey: "Members of the human glyceraldehyde-3-phosphate dehydrogenase-related gene family map to dispersed chromosomal locations." in: Genomics, Vol. 5, Issue 2, pp. 209-14, (1989) (PubMed).

  • 抗原

    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

    别名

    Glyceraldehyde-3-phosphate dehydrogenase(GAPDH)

    背景

    Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme is thought to be a tetramer of identical chains. Several highly homologous glyceraldehyde-3-phosphate dehydrogenase (GAPD)-related sequences have been identified previously in human DNA by Southern blot analysis. Protein studies have identified only a single expressed locus for this major glycolytic enzyme, and this maps to chromosome 12p13. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a critical regulator of CICD, it mediates an elevation in glycolysis and enhanced autophagy that cooperate to protect cells from CICD.
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