This anti-Estrogen Receptor alpha antibody is a 兔 多克隆 antibody detecting Estrogen Receptor alpha in IHC. Suitable for 人. This Primary Antibody has been cited in 2+ publications.
Estrogen Receptor- (Ab-167) antibody detects endogenous levels of total Estrogen Receptor- protein.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
免疫原
The antiserum was produced against synthesized non-phosphopeptide derived from human Estrogen Receptor- around the phosphorylation site of serine167 (L-A-SP-T-N).
ESR1
适用: 人, 小鼠, 大鼠
WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
WB: 1:500~1:1000 IHC: 1:50~1:100.
限制
仅限研究用
状态
Liquid
浓度
100ug/100ul.
缓冲液
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
Shah, Rowan: "The Src kinase pathway promotes tamoxifen agonist action in Ishikawa endometrial cells through phosphorylation-dependent stabilization of estrogen receptor (alpha) promoter interaction and elevated steroid receptor coactivator 1 activity." in: Molecular endocrinology (Baltimore, Md.), Vol. 19, Issue 3, pp. 732-48, (2005) (PubMed).
Campbell, Bhat-Nakshatri, Patel, Constantinidou, Ali, Nakshatri: "Phosphatidylinositol 3-kinase/AKT-mediated activation of estrogen receptor alpha: a new model for anti-estrogen resistance." in: The Journal of biological chemistry, Vol. 276, Issue 13, pp. 9817-24, (2001) (PubMed).