This anti-Estrogen Receptor alpha antibody is a 兔 多克隆 antibody detecting Estrogen Receptor alpha in IHC. Suitable for 人 和 小鼠. This Primary Antibody has been cited in 2+ publications.
Estrogen Receptor- (Ab-106) antibody detects endogenous levels of total Estrogen Receptor- protein.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
免疫原
The antiserum was produced against synthesized non-phosphopeptide derived from human Estrogen Receptor- around the phosphorylation site of serine 106 (S-P- SP-P-L).
ESR1
适用: 人, 小鼠, 大鼠
WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
WB: 1:500~1:1000 IHC: 1:50~1:100.
限制
仅限研究用
状态
Liquid
浓度
100ug/100ul.
缓冲液
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
Medunjanin, Hermani, De Servi, Grisouard, Rincke, Mayer: "Glycogen synthase kinase-3 interacts with and phosphorylates estrogen receptor alpha and is involved in the regulation of receptor activity." in: The Journal of biological chemistry, Vol. 280, Issue 38, pp. 33006-14, (2005) (PubMed).
Rogatsky, Trowbridge, Garabedian: "Potentiation of human estrogen receptor alpha transcriptional activation through phosphorylation of serines 104 and 106 by the cyclin A-CDK2 complex." in: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22296-302, (1999) (PubMed).