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CD8 抗体

This anti-CD8 antibody is a 小鼠 单克隆 antibody detecting CD8 in FACS, IF, IHC (fro), ELISA 和 IHC (af). Suitable for 人. This Primary Antibody has been cited in 3+ publications.
产品编号 ABIN965819
发货至: 中国

Quick Overview for CD8 抗体 (ABIN965819)

抗原

See all CD8 抗体
CD8

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD8 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunohistochemistry (Acetone-fixed) (IHC (af))

克隆位点

B362 (RIV 11)
  • 特异性

    This antibody reacts with a 32 kD molecule present on human suppressor/cytotoxic T-cells. These antibodies also react with sinus-lining cells in the human spleen.

    纯化方法

    Purified.

    免疫原

    Human peripheral lymphocytes.

    亚型

    IgG1
  • 应用备注

    Immunofluorescence: 5-20 ug/mL.
    Flow cytometry: 1-5 ug/10^6 cells.
    ELISA: 0.2-1.0 ug/mL.
    Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
    Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections.

    说明

    Recommended Positive Control: Tonsil and Lymph Node

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.4 mg/ml.

    缓冲液

    50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C
  • Barber, Dasgupta, Schlossman, Trevillyan, Rudd: "The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 9, pp. 3277-81, (1989) (PubMed).

    Jonker, Nooij, den Butter, van Lambalgen, Fuccello: "Side effects and immunogenicity of murine lymphocyte-specific monoclonal antibodies in subhuman primates." in: Transplantation, Vol. 45, Issue 4, pp. 677-82, (1988) (PubMed).

    Reinherz, Kung, Goldstein, Schlossman: "A monoclonal antibody reactive with the human cytotoxic/suppressor T cell subset previously defined by a heteroantiserum termed TH2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 124, Issue 3, pp. 1301-7, (1980) (PubMed).

  • 抗原

    CD8

    基因ID

    925
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