(1 reference)-
- 抗原
- B-Cells
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
-
应用范围
- Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- This antibody defines the antigen which appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD 37 and cym. It is expressed on resting B cells and then reappears and persists in the cytoplasm and on the cell surface until cytoplasmic Ig appears. It is a useful antibody for studying B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50% of cells). This antibody does not label neoplasms of T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again.
- 纯化方法
- Concentrated.
- 免疫原
- Human B cell line Daudi.
- 克隆位点
- B381 (IPO10)
- 亚型
- IgG3
-
anti-B-Cells antibody (FITC)
适用: 人 FACS 宿主: 小鼠 Monoclonal FMC7 FITC
-
- 应用备注
- Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:60 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique (see instructions below) is strongly recommended for consistent and reproducible results.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.3 mg/ml.
- 缓冲液
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
-
-
: "Adrenergic receptor research: recent developments." in: Progress in drug research. Fortschritte der Arzneimittelforschung. Progrès des recherches pharmaceutiques, Vol. 33, pp. 151-67, (1990) (PubMed).
-
: "Adrenergic receptor research: recent developments." in: Progress in drug research. Fortschritte der Arzneimittelforschung. Progrès des recherches pharmaceutiques, Vol. 33, pp. 151-67, (1990) (PubMed).
-
- 抗原
- B-Cells
- 别名
- B Cell
-
You are here:
