PRDM1 抗体 (C-Term)
Quick Overview for PRDM1 抗体 (C-Term) (ABIN964913)
抗原
See all PRDM1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-b52
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Supplier
- Rockland
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原理
- PRDM1 BLIMP1 Antibody
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交叉反应 (详细)
- This affinity purified antibody is directed against human BLIMP1 protein.
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产品特性
- Synonyms: rabbit anti-Blimp-1 antibody, rabbit anti-PRDM-1 antibody, anti-PRDM1/BLIMP1 antibody, PR domain zinc finger protein 1, PR domain-containing protein 1, Beta-interferon gene positive regulatory domain I-binding factor, BLIMP-1, Beta-lymphocyte-induced maturation protein 1, positive regulatory domain I-binding factor 1, PRDI-BF1, PRDI-binding factor 1, BLIMP1, BLIMP 1, PRDM1
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Blimp1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of human, mouse, and rat PRDM1/BLIMP1.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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应用备注
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Immunohistochemistry Dilution: 5 μg/mL
Application Note: This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~88 kDa in size corresponding to BLIMP1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000
Immunoprecipitation Dilution: 5-10 μg/mL
ELISA Dilution: 1:20,000 - 1:40,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.15 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Blimp1 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))
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别名
- PRDM1
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背景
- Background: Anti-Blimp1 is ideal for Immunology, Epigenetic and Nuclear Signaling research. PRDM1/Blimp1 (PR domain zinc finger protein 1, PR domain-containing protein 1, Beta-interferon gene positive regulatory domain I-binding factor, BLIMP-1, Positive regulatory domain I-binding factor 1, PRDI-binding factor 1, or PRDI-BF1, or Beta-lymphocyte-induced maturation protein 1) is one of several transcription factors which drives the maturation of B-lymphocytes into Ig secreting cells. In the nucleus, PRDM1/BLIMP1 functions as a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Transcription of the PRDM1 gene is increased upon virus induction. PRDM1/Blimp1 also interacts with PRMT5. Two alternatively spliced transcript variants that encode different isoforms have been reported.
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基因ID
- 639, 25008939
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UniProt
- Q60636
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途径
- Regulation of Muscle Cell Differentiation
抗原
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