Interleukin 17a 抗体
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- 抗原 See all Interleukin 17a (IL17A) 抗体
- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This Interleukin 17a antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Multiplex Assay (MA)
- Supplier Product No.
- 210-501-b32s
- Supplier
- Rockland
- 原理
- Mouse IL-17A Antibody
- 交叉反应 (详细)
- This antibody is specific for mouse IL-17A protein.
- 产品特性
- Synonyms: rat anti-IL-17A antibody, rat anti-interleukin17A antibody, Interleukin-17A cytokine, IL-17A, IL-17, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8
- 纯化方法
- This monoclonal antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This Mouse IL-17A monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E.coli) followed by hybridoma development.
Immunogen Type: Recombinant Protein
- 克隆位点
- 20B4-G10-F5
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product IL17A Primary Antibody
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- 应用备注
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Flow Cytometry Dilution: 1:500
Application Note: IL-17 A antibody has been tested for use in western blotting, Flow Cytometry and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23 kDa in size corresponding to the mature mouse IL-17A protein, a non-glycosylated polypeptide chain consisting of 207 amino acids, by western blotting in appropriate cell lysate or extract.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:10,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2.94 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store IL-17A antibody at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
- 别名
- IL17a (IL17A 产品)
- 背景
- Background: Mouse Interleukin-17A (IL-17A), also known as CTLA-8, is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Mouse IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58 % identity between the amino acid sequence of human and mouse IL-17A. Anti-IL-17A antibody is ideal for investigators involved in Immunology research.
- 基因ID
- 16171
- NCBI登录号
- NP_034682
- UniProt
- Q62386
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