CCL20 抗体 (Biotin)
Quick Overview for CCL20 抗体 (Biotin) (ABIN964794)
抗原
See all CCL20 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 210-406-b51
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Supplier
- Rockland
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原理
- MIP-3 alpha Antibody Biotin Conjugated
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交叉反应 (详细)
- In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse MIP-3α in cell supernatants and certain body fluids.
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产品特性
- Synonyms: rabbit anti-MIP-3α biotin conjugated antibody, CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1
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纯化方法
- This Protein A purified antibody was heated to 56°C for 30 minutes.
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免疫原
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Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse MIP-3alpha protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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应用备注
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Immunohistochemistry Dilution: 1:1,000-1:5,000
Application Note: This purified antibody has been tested by dot blot and is suitable for use in ELISA and western blotting. By western blot a band approximately 10.7 kDa in size corresponding to mouse MIP-3α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:2,000-1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))
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别名
- Ccl20
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背景
- Background: MIP-3α (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3α inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3α also possesses antibacterial activity against E.coli and S.aureus. MIP-3α is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
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基因ID
- 20297
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NCBI登录号
- NP_058656
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UniProt
- O89093
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途径
- The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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