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IL-7 抗体 (Biotin)

IL7 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal Biotin
Rockland
产品编号 ABIN964779
Supplier Product No.: 209-406-b94
发货至: 中国
  • 抗原 See all IL-7 (IL7) 抗体
    IL-7 (IL7) (Interleukin 7 (IL7))
    适用
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    宿主
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    克隆类型
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    标记
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    This IL-7 antibody is conjugated to Biotin
    应用范围
    • 65
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    209-406-b94
    Supplier
    Rockland
    原理
    IL-7 Antibody Biotin Conjugated
    产品特性
    Synonyms: rabbit anti-IL-7 biotin conjugated antibody, rabbit anti-interleukin-7 antibody, IL7, IL-7 Interleukin 7, Interleukin-7
    纯化方法
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56 °C for 30 minutes.
    免疫原

    Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.

    Immunogen Type: Recombinant Protein

    亚型
    IgG
    Top Product
    Discover our top product IL7 Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000-1:5,000

    Application Note: This purified antibody has been tested in western blotting and suitable for ELISA. By western blot a band approximately 17.4 kDa in size corresponding to human IL-7 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: 1:2,000-1:10,000

    ELISA Dilution: 1:20,000-1:100,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    IL-7 (IL7) (Interleukin 7 (IL7))
    别名
    IL7 (IL7 产品)
    背景
    Background: Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.
    基因ID
    3574
    NCBI登录号
    NP_000871
    UniProt
    P13232
    途径
    JAK/STAT Signaling
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