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- 抗原 See all IL-7 (IL7) 抗体
- IL-7 (IL7) (Interleukin 7 (IL7))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IL-7 antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)
- Supplier Product No.
- 209-403-b94
- Supplier
- Rockland
- 原理
- IL-7 Antibody Peroxidase Conjugated
- 产品特性
- Synonyms: rabbit anti-IL-7 peroxidase conjugated antibody, rabbit anti-interleukin-7 antibody, IL7, IL-7 Interleukin 7, Interleukin-7
- 纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56 °C for 30 minutes.
- 免疫原
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Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product IL7 Primary Antibody
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anti-Interleukin 7 (IL7) (AA 1-177) antibody
IL7 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) antibodyIL7 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (AA 26-177) antibodyIL7 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (AA 26-177) antibodyIL7 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (AA 51-154) antibodyIL7 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (AA 26-177) antibodyIL7 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Interleukin 7 (IL7) antibodyIL7 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (C-Term) antibodyIL7 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) antibody (Biotin)IL7 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Interleukin 7 (IL7) antibody (Biotin)IL7 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
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- 应用备注
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Immunohistochemistry Dilution: 1:500-1:2,500
Application Note: This purified antibody has been tested in western blotting and ELISA. By western blot a band approximately 17.4 kDa in size corresponding to human IL-7 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:1,000-1:5,000
ELISA Dilution: 1:10,000-1:50,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IL-7 (IL7) (Interleukin 7 (IL7))
- 别名
- IL7 (IL7 产品)
- 背景
- Background: Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.
- 基因ID
- 3574
- NCBI登录号
- NP_000871
- UniProt
- P13232
- 途径
- JAK/STAT Signaling
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