IL-3 抗体
Quick Overview for IL-3 抗体 (ABIN964755)
抗原
See all IL-3 抗体适用
宿主
克隆类型
标记
应用范围
-
-
Supplier Product No.
- 209-401-b92
-
Supplier
- Rockland
-
原理
- IL-3 Antibody
-
产品特性
- Synonyms: rabbit anti-IL-3 antibody, rabbit anti-Interleukin 3 antibody, IL3, interleukin-3, Burst promoting activity, Colony stimulating factor, Eosinophil colony stimulating factor, Hematopoietic growth factor, IL-3, Hematopoietic growth factor, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor
-
纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56 °C for 30 minutes.
-
免疫原
-
Immunogen: IL-3 antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein.
Immunogen Type: Recombinant Protein
-
亚型
- IgG
-
-
anti-Interleukin 3 (IL-3) antibody
IL-3 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 101-166) antibodyIL-3 适用: 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 20-152) antibodyIL-3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) antibodyIL-3 适用: 大鼠 WB, ELISA, Neut 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 33-166) antibodyIL-3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 1-152) antibodyIL-3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 1-152) antibodyIL-3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (Internal Region) antibodyIL-3 适用: 人, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) antibody (Biotin)IL-3 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Interleukin 3 (IL-3) antibody (Biotin)IL-3 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
-
-
应用备注
-
Application Note: This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 15 kDa in size corresponding to native human IL-3 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:10,000
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None -
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store anti-IL-3 antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- IL-3 (Interleukin 3 (IL-3))
-
别名
- IL3
-
背景
- Background: The pleiotropic IL3 (Interleukin 3) is a 15 kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis. Anti-IL-3 antibody is ideal for investigators involved in Cancer and Immunology research.
-
基因ID
- 3562
-
NCBI登录号
- NP_000579
-
UniProt
- P08700
-
途径
- JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
抗原
-
