Fluorescein 抗体
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北京 101111
Quick Overview for Fluorescein 抗体 (ABIN964651)
抗原
片段
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 700-101-096
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Supplier
- Rockland
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原理
- F(Ab')2 Fluorescein Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated BSA.
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产品特性
- Synonyms: goat F(ab')2 anti-Fluorescein Antibody, goat F(ab')2 anti-FITC Antibody
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Fluorescein conjugated to Goat IgG
Immunogen Type: Other
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亚型
- IgG
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anti-Fluorescein antibody
WB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated
anti-Fluorescein antibodyWB, ELISA, IHC, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Fluorescein antibodyWB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:200 - 1:1,000
ELISA Dilution: 1:2,000 - 1:8,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Fluorescein
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物质类
- Chemical
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背景
- Background: F(ab')2 Anti-Fluorescein Antibody generated in goat detects fluorescein. It is an organic dye used as a fluorescent tracer in many application including cellular staining.
抗原
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