Vitamin D Receptor 抗体 (AA 89-105)
Quick Overview for Vitamin D Receptor 抗体 (AA 89-105) (ABIN962160)
抗原
See all Vitamin D Receptor (VDR) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 89-105
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品牌
- IHC-plus™
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特异性
- Recognizes both the occupied and unoccupied forms of VDR. Does not cross-react with estrogen and glucocorticoid receptors. In mammals, recognizes a protein of ~53kD which is identified as vitamin D Receptor (VDR). In avians, VDR appears as a doublet of 58 and 60kD. Species cross-reactivity: Human, porcine, mouse, rat, hamster, frog, chicken and fish.
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纯化方法
- Protein G purified
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免疫原
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Vitamin D receptor protein from chicken intestine (53kD). Cellular Localization: Nuclear. Epitope: 89-105.
Type of Immunogen: Purified protein -
亚型
- IgG2b
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应用备注
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Approved: IHC-P (10 μg/mL), WB (1 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: LNCaP and HeLa cells. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.2 % BSA, 0.09 % sodium azide. Sourced in Ascites.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.
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- Vitamin D Receptor (VDR)
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别名
- Vitamin D Receptor / VDR
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物质类
- Chemical
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背景
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Name/Gene ID: VDR
Subfamily: NR1 Thyroid hormone-like
Family: NHR
Synonyms: VDR, NR1I1, Vitamin D3 receptor, Vitamin D receptor -
基因ID
- 7421
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UniProt
- P11473
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途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
抗原
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