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Vitamin D Receptor 抗体 (AA 89-105)

The 大鼠 单克隆 anti-Vitamin D Receptor antibody (ABIN962160) specifically detects Vitamin D Receptor in WB 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN962160
发货至: 中国
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Quick Overview for Vitamin D Receptor 抗体 (AA 89-105) (ABIN962160)

抗原

See all Vitamin D Receptor (VDR) 抗体
Vitamin D Receptor (VDR)

适用

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宿主

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大鼠

克隆类型

  • 96
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单克隆

标记

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This Vitamin D Receptor antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    AA 89-105

    特异性

    Recognizes both the occupied and unoccupied forms of VDR. Does not cross-react with estrogen and glucocorticoid receptors. In mammals, recognizes a protein of ~53kD which is identified as vitamin D Receptor (VDR). In avians, VDR appears as a doublet of 58 and 60kD. Species cross-reactivity: Human, porcine, mouse, rat, hamster, frog, chicken and fish.

    纯化方法

    Protein G purified

    免疫原

    Vitamin D receptor protein from chicken intestine (53kD). Cellular Localization: Nuclear. Epitope: 89-105.

    Type of Immunogen: Purified protein

    亚型

    IgG2b
  • 应用备注

    Approved: IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: LNCaP and HeLa cells.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, pH 7.4, 0.2 % BSA, 0.09 % sodium azide. Sourced in Ascites.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Long term: -20°C
    Short term: +4°C. Avoid repeat freeze-thaw cycles.
  • 抗原

    Vitamin D Receptor (VDR)

    别名

    Vitamin D Receptor / VDR

    物质类

    Chemical

    背景

    Name/Gene ID: VDR
    Subfamily: NR1 Thyroid hormone-like
    Family: NHR

    Synonyms: VDR, NR1I1, Vitamin D3 receptor, Vitamin D receptor

    基因ID

    7421

    UniProt

    P11473

    途径

    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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