PUMA 抗体 (AA 2-16)
Quick Overview for PUMA 抗体 (AA 2-16) (ABIN959855)
抗原
See all PUMA (BBC3) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 2-16
-
品牌
- IHC-plus™
-
特异性
- Recognizes the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2. PUMA is a 193 amino acid ~20 kD pro-apoptotic member of the BCL-2 family. Bcl-2 binding component 3 is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53. Four isoforms of PUMA have been described due to alternative splicing. Is expected to recognize the canonical isofom 1, also known as PUMA alpha, it is not expected to recognize any of the other isoforms of PUMA.
-
预测反应
- Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%) Opossum (87%).
-
纯化方法
- Purified
-
免疫原
-
Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human PUMA alpha. Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%), Opossum (87%).
Type of Immunogen: Synthetic peptide -
亚型
- IgG
-
-
anti-BCL2 Binding Component 3 (BBC3) (AA 131-180) antibody
BBC3 适用: 人, 大鼠, Pig WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibodyBBC3 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibodyBBC3 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB01353 unconjugated
anti-BCL2 Binding Component 3 (BBC3) (BH3 Domain), (C-Term) antibodyBBC3 适用: 人, 小鼠 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 186-199) antibodyBBC3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibodyBBC3 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 98-152) antibodyBBC3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5D10 unconjugated
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibodyBBC3 适用: 人, 小鼠 WB, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 109-137) antibodyBBC3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibodyBBC3 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Approved: IHC, IHC-P (2.5 μg/mL), WB (2 - 4 μg/mL)
Usage: Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Histology Positive Control Tissue: Human breast carcinoma. Western Blot: detects a band of approximately 23kD in K562 cell lysates. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, 0.02 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- avoid freeze thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
-
-
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
-
别名
- PUMA
-
背景
-
Name/Gene ID: BBC3
Family: Apoptosis
Synonyms: BBC3, Bcl-2-binding component 3, BCL2 binding component 3, JFY-1, JFY1, PUMA -
基因ID
- 27113
-
UniProt
- Q9BXH1
-
途径
- p53 Pathway, Positive Regulation of Endopeptidase Activity
抗原
-
