PF4 抗体 (Biotin)
Quick Overview for PF4 抗体 (Biotin) (ABIN954176)
抗原
See all PF4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody is specific for Human Platelet Factor IV/heparin complexes. AM31851BT-N (clone KKO), unlike AM31850BT-N (clone RTO) which binds PF4 alone, is specific for PF4/heparin complexes and is seen to have similar binding properties as auto-antibodies found in HIT/HITT.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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免疫原
- Human Factor IV purified from the supernatant of Thrombin activated platelets.
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亚型
- IgG2b
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anti-Platelet Factor 4 (PF4) (AA 31-101) antibody
PF4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Platelet Factor 4 (PF4) (AA 31-101) antibodyPF4 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Platelet Factor 4 (PF4) antibodyPF4 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Platelet Factor 4 (PF4) (AA 25-105) antibodyPF4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Platelet Factor 4 (PF4) (AA 31-101) antibodyPF4 适用: 人 WB, IP, ELISA 宿主: 小鼠 Monoclonal 3F6 unconjugated
anti-Platelet Factor 4 (PF4) (AA 1-101) antibodyPF4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Platelet Factor 4 (PF4) antibodyPF4 适用: 人 IHC, ELISA, IHC (p) 宿主: 绵羊 Polyclonal unconjugated
anti-Platelet Factor 4 (PF4) antibodyPF4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Platelet Factor 4 (PF4) (AA 30-105) antibodyPF4 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Platelet Factor 4 (PF4) (AA 30-105) antibodyPF4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA: Use at 0.08 μg/mL following coating plate with PF4/ heparin complex. This can be done by incubating PF4 with an excess of heparin (100-200 U/mL), while coating the plate.
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PF4 (Platelet Factor 4 (PF4))
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别名
- Platelet Factor 4 / PF4
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背景
- Platelet Factor IV (PF4) is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high affinity to heparin and plays a role in inflammation and wound repair. PF4 is a chemoattractant for neutrophils, monocytes and fibroblasts and has been reported to be an immunologic regulator that inhibits suppressor T-cell activity. Heparin-Induced Thrombocytopenia/Thrombosis (HIT/HITT) is a life-threatening complication that manifests itself in a small population of patients exposed to intravenous-heparin. It is characterized by the production of PF4/heparin auto-antibodies. These auto-antibodies bind the PF4/heparin complexes and subsequently bind the FCGIIa receptor on the platelets surface through their Fc region. This activates the platelets and can initiate clot formation.Synonyms: C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4
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基因ID
- 5196
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NCBI登录号
- NP_002610
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UniProt
- P02776
抗原
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