ATG12 抗体
Quick Overview for ATG12 抗体 (ABIN95224)
抗原
See all ATG12 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-401-437
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Supplier
- Rockland
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原理
- APG12 Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
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产品特性
- Synonyms: rabbit anti-APG12 antibody, APG-12, APG 12, ATG-12, ATG 12, APG12-like antibody, APG12L antibody, ATG12 antibody, ATG12 autophagy related 12 homolog (S. cerevisiae) antibody, ATG12 autophagy related 12 homolog antibody, Autophagy 12 antibody, Autophagy-related protein 12 antibody, HAPG12 antibody
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纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast APG12 protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Autophagy Related 12 (ATG12) (N-Term) antibody
ATG12 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 猴, 小鸡, 兔, Pig, Cat, 驴, 山羊, Hamster, 绵羊, Avian WB, IF 宿主: 山羊 Polyclonal unconjugated
anti-Autophagy Related 12 (ATG12) antibodyATG12 适用: 人 WB, ELISA, IHC, IF 宿主: 小鼠 Monoclonal 43CT73-3-5-5-4 unconjugated
anti-Autophagy Related 12 (ATG12) (AA 101-187) antibodyATG12 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-74) antibodyATG12 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-30), (N-Term) antibodyATG12 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7475 unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-50) antibodyATG12 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 12 (ATG12) antibodyATG12 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Autophagy Related 12 (ATG12) (AA 79-128) antibodyATG12 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 斑马鱼, 猴, 小鸡, 兔, Pig, Bat, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-74) antibodyATG12 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H8 unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-140) antibodyATG12 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F9 unconjugated
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应用备注
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Application Note: This purified polyclonal antibody reacts yeast APG12 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 5.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- ATG12 (Autophagy Related 12 (ATG12))
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别名
- ATG12
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背景
- Background: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. In yeast, autophagy, the delivery of cytoplasmic components to the lysosome/vacuole for degradation, requires a ubiquitin-like protein conjugation system, in which Apg12 is covalently bound to Apg12-Apg5 and Apg16.
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基因ID
- 852518, 6319694
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UniProt
- P38316
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途径
- Autophagy
抗原
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