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APP 抗体

Name: APP 抗体
SKU: ABIN95037
Availability: OutOfStock
Rating: 5 (2 reviews)

This anti-APP antibody is a 兔多克隆 antibody detecting APP in WB, ELISA, IHC, IF 和 FM. Suitable for 人和小鼠. Independently validated for use in Unfolding Profile, Immunohistochemistry. This Primary Antibody has been cited in 1 publication.

Immunohistochemistry validation image for anti-Amyloid beta (A4) Precursor Protein (APP) antibody (ABIN95037) (APP 抗体)

IHC

Western Blot of Rabbit anti-Beta Amyloid antibody. (APP 抗体)

Immunofluorescence microscopy of Rabbit Anti-Beta Amyloid antibody using HeLa cells fixed with MeOH. (APP 抗体)

Immunohistochemistry with anti-beta amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C). (APP 抗体)

IHC

ELISA

Human Heart (formalin-fixed, paraffin-embedded) stained with Anti-Beta Amyloid Antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. (APP 抗体)

IHC

Immunofluorescence (IF) image for anti-Amyloid beta (A4) Precursor Protein (APP) antibody (ABIN95037) (APP 抗体)

9 images

(1 reference)

(2 validations)

产品编号 ABIN95037

Supplier Product No.: 600-401-253

发货至: 中国

Datasheet as PDF

Quick Overview for APP 抗体 (ABIN95037)

抗原

See all APP 抗体

APP (Amyloid beta (A4) Precursor Protein (APP))

适用

人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This APP antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Discover our top product APP Primary Antibody

Supplier Product No.

600-401-253

Supplier

Rockland

原理

Beta Amyloid Antibody

交叉反应（详细）

This affinity purified antibody is directed against extracellular region of beta amyloid and is useful in determining its presence in various assays.

产品特性

Synonyms: rabbit anti-Beta Amyloid Antibody, ß-amyloid, Amyloid beta A4 protein, Alzheimer disease amyloid protein, Beta amyloid, A-beta, ABPP, APPI, Beta-amyloid precursor protein

纯化方法

affinity purified antibody

过滤

Sterile filtered

免疫原

Immunogen: This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an extracellular region of human beta amyloid conjugated to KLH using maleimide.
Immunogen Type: Conjugated Peptide

亚型

IgG
anti-Amyloid beta (A4) Precursor Protein (APP) (C-Term) antibody
APP 适用: 人 WB, IF, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody
APP 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Amyloid beta (A4) Precursor Protein (APP) (AA 18-270) antibody
APP 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Amyloid beta (A4) Precursor Protein (APP) (C-Term) antibody
APP 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Amyloid beta (A4) Precursor Protein (APP) antibody
KO Validated APP 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Amyloid beta (A4) Precursor Protein (APP) (AA 381-480) antibody
APP 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Amyloid beta (A4) Precursor Protein (APP) (pThr668) antibody
APP 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Amyloid beta (A4) Precursor Protein (APP) antibody
APP 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Amyloid beta (A4) Precursor Protein (APP) (C-Term) antibody
APP 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated

anti-Amyloid beta (A4) Precursor Protein (APP) (AA 18-286) antibody
APP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry Dilution: 1:50-1:200
Application Note: Affinity purified anti-beta amyloid has been tested by ELISA, IHC, WB, and IF. A 45.8 kDa band is detected in western blot using whole tissue extracts and lysates from mouse and human. In general, we recommend the use of 4 % PFA or 10 % formalin for fixation of tissues with IHC-paraffin or IHC-frozen application of this antibody.
Western Blot Dilution: 1:1,000-1:5000
ELISA Dilution: 1:10,000 - 1:30,000
IF Microscopy Dilution: 1:50-1:200
Other: User Optimized

限制

仅限研究用
生效 #104082 (Unfolding Profile)

by

NanoTemper Technologies

No.

#104082

日期

2019.07.23

抗原

ABETA

Lot Number

Method validated

Unfolding Profile

Positive Control

ABIN1043866

Negative Control

Notes

Passed. ABIN1043866 showed T_i at 83.8°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

Validation Images

Unfolding profile of ABIN1043866. The fluorescence signal is plotted against temperature. The vertical line indicates the Ti at 83.8 °C.
Full Methods
Primary Antibody

Secondary Antibody

Full Protocol

Load the undiluted sample into Tycho capillary (NanoTemper Technologies, TY-C001).
Run Tycho measurement.

Experimental Notes

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.
生效 #104424 (Immunohistochemistry)

by

Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.

#104424

日期

2022.05.12

抗原

beta Amyloid

Lot Number

42581

Method validated

Immunohistochemistry

Positive Control

Adult (24 months) mouse brain

3-month-old Taconic mouse Model 1349 brain fixed in 4% paraformaldehyde

Negative Control

We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.

Notes

The beta Amyloid antibody (Amyloid beta) (N-Term) ABIN95037 works in IHC-P, especially at higher concentrations (1:20 and 1:50), and without the use of any antigen retrieval treatment.

Validation Images

Staining of beta-amyloid positive cells in the adult mouse brain using ABIN95037 at 40x magnification.
Full Methods
Primary Antibody

ABIN95037

Secondary Antibody

goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418)

Full Protocol

Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2 h at RT.
Wash, dehydrate, and embed samples in paraffin wax.
Wash several times with 0.01 M PBS.
Cut intestines and brain with a microtome into 6µm sections and mount on glass slides.
After paraffin removal, incubate sections for 1 h at RT in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
Incubate sections with primary anti-beta amyloid (N-Term) (antibodies Online, ABIN95037, lot 42581) diluted 1:20, 1:50, 1:100, and 1:200 in 0.1 M PBS-BSA-PLL ON at RT.
Wash sections 3x 5 min with 0.01 M PBS.
Incubate sections with secondary goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418) diluted 1:500 in 0.1 M PBS for 1 h at RT.
Wash sections 3x 5 min with 0.01M PBS.
Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.

Experimental Notes

Antigen retrieval treatment was also tested. In this case, sections were processed for microwave antigen retrieval for 10 min (95-100 °C) in 10 mM sodium citrate buffer (pH 6.0). After 20 min of spontaneous cooling, sections were washed twice for 5 min with distilled water and for 5 min with PBS.
状态

Liquid

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Frenkel, Balass, Solomon: "N-terminal EFRH sequence of Alzheimer's beta-amyloid peptide represents the epitope of its anti-aggregating antibodies." in: Journal of neuroimmunology, Vol. 88, Issue 1-2, pp. 85-90, (1998) (PubMed).
抗原

APP (Amyloid beta (A4) Precursor Protein (APP))

别名

APP

背景

Background: Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase. Anti-Beta Amyloid Antibody is useful for researchers interested in TLR signaling and Alzheimer's research.

基因ID

351

NCBI登录号

NP_000475

UniProt

P05067

途径

Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour