Intestinal Alkaline Phosphatase 抗体
(1 reference)
Quick Overview for Intestinal Alkaline Phosphatase 抗体 (ABIN94935)
抗原
See all Intestinal Alkaline Phosphatase (ALPI) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4135-0100
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Supplier
- Rockland
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原理
- Alkaline Phosphatase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].
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产品特性
- Synonyms: rabbit anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody
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纯化方法
- Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Alkaline Phosphatase [Calf Intestine]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Intestinal Alkaline Phosphatase (ALPI) (AA 397-458) antibody
ALPI 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 3B10E7 unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 397-458) antibodyALPI 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 3B10E7 unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 74-162) antibodyALPI 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A8 unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) antibodyALPI 适用: 人 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 20-333) antibodyALPI 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 26-500) antibodyALPI 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 397-458) antibodyALPI 适用: 人 ELISA, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 3B10E7 unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 20-240) antibodyALPI 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 53-102), (pSer87) antibodyALPI 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) antibodyALPI 适用: Cow WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Alkaline Phosphatase has been tested against 1.0 μg of Alkaline Phosphatase [Calf intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Cellular Plasticity of Defa4Cre-Expressing Paneth Cells in Response to Notch Activation and Intestinal Injury." in: Cellular and molecular gastroenterology and hepatology, Vol. 7, Issue 3, pp. 533-554, (2019) (PubMed).
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: "Cellular Plasticity of Defa4Cre-Expressing Paneth Cells in Response to Notch Activation and Intestinal Injury." in: Cellular and molecular gastroenterology and hepatology, Vol. 7, Issue 3, pp. 533-554, (2019) (PubMed).
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- Intestinal Alkaline Phosphatase (ALPI)
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别名
- Calf Intenstinal Alkaline Phosphatase
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背景
- Background: In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Alkaline Phosphatase catalytic activity is phosphate monoester + H2O = an alcohol + phosphate.
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基因ID
- 280993
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UniProt
- P19111
抗原
-
