ATP1A1 抗体
Quick Overview for ATP1A1 抗体 (ABIN94710)
抗原
See all ATP1A1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-9155
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Supplier
- Rockland
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原理
- ATPase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney].
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产品特性
- Synonyms: chicken anti-ATPase Antibody, Sodium/potassium-transporting ATPase
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纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: ATPase, Sodium, Potassium [Canine Kidney]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Sodium Potassium ATPase, alpha1 (ATP1A1) (N-Term) antibody
ATP1A1 适用: 人 WB 宿主: 山羊 Polyclonal unconjugated
anti-Sodium Potassium ATPase, alpha1 (ATP1A1) (N-Term) antibodyATP1A1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sodium Potassium ATPase, alpha1 (ATP1A1) antibodyATP1A1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Sodium Potassium ATPase, alpha1 (ATP1A1) (pTyr10) antibodyATP1A1 适用: 人 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Sodium Potassium ATPase, alpha1 (ATP1A1) (AA 493-660) antibodyATP1A1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C206 unconjugated
anti-Sodium Potassium ATPase, alpha1 (ATP1A1) (AA 493-660) antibodyATP1A1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sodium Potassium ATPase, alpha1 (ATP1A1) (AA 225-407) antibodyATP1A1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Sodium Potassium ATPase, alpha1 (ATP1A1) (pSer16) antibodyATP1A1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sodium Potassium ATPase, alpha1 (ATP1A1) (AA 1-100) antibodyATP1A1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sodium Potassium ATPase, alpha1 (ATP1A1) (Ser23) antibodyATP1A1 适用: 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-ATPase Na+ K+ has been assayed against 1.0 μg of ATPase, Sodium, Potassium in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit) code #603-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))
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别名
- ATP1A1
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背景
- Background: Sodium/potassium-transporting ATPase subunit alpha-1 is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
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基因ID
- 403992
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NCBI登录号
- NP_001003306
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UniProt
- P50997
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途径
- Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process
抗原
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