SIT1 抗体
This anti-SIT1 antibody is a 小鼠 单克隆 antibody detecting SIT1 in WB, IP 和 ICFC. Suitable for 人. This Primary Antibody has been cited in 2+ publications.
(2 references)Quick Overview for SIT1 抗体 (ABIN94465)
抗原
See all SIT1 抗体
SIT1
(Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This SIT1 antibody is un-conjugated
应用范围
Western Blotting (WB), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC)
克隆位点
SIT-01
-
-
原理
- Anti-SIT Purified
-
特异性
- The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with murine SIT.
-
交叉反应 （详细）
- Human
-
纯化方法
- Purified by protein-A affinity chromatography.
-
纯度
- > 95 % (by SDS-PAGE)
-
免疫原
- Bacterially produced recombinant intracellular fragment of human SIT.
-
亚型
- IgG1
-
-
anti-Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1) antibody (PE)
SIT1 适用: 人 ICFC 宿主: 小鼠 Monoclonal SIT-01 PE
-
-
应用备注
-
Flow cytometry: Recommended dilution: 1-5 μg/mL, intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions. -
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Do not freeze.
-
储存条件
- 4 °C
-
储存方法
- Store at 2-8°C. Do not freeze.
-
-
-
- SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))
-
别名
- SIT
-
背景
- Signaling threshold regulating transmembrane adapt,SIT is a 21 kDa transmembrane adaptor protein expressed exclusively in lymphoid organs. It acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.,SIT1
-
基因ID
- 27240
-
UniProt
- Q9Y3P8
抗原
-
You are here:
