LAT2 抗体 (Cytoplasmic Domain)
(5 references)Quick Overview for LAT2 抗体 (Cytoplasmic Domain) (ABIN94439)
抗原
See all LAT2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 91-243, Cytoplasmic Domain
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原理
- Anti-NTAL Purified
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特异性
- The antibody NAP-07 reacts with an intracellular epitope of Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein associating with lipid raft components in the plasma membrane of B lymphocytes, NK cells and myeloid cells.
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交叉反应 （详细）
- Mouse, Human, Other not tested
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Recombinant cytoplasmic domain (aa 91-243) of human NTAL.
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亚型
- IgG1
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anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 30-209) antibody
LAT2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibodyLAT2 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-243), (Cytoplasmic Domain) antibody (PE)LAT2 适用: 人, 小鼠 ICFC 宿主: 小鼠 Monoclonal NAP-07 PE
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 32-192) antibodyLAT2 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 124-203), (Cytoplasmic Domain) antibodyLAT2 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 1-20) antibodyLAT2 适用: 人 IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibodyLAT2 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (C-Term) antibodyLAT2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-244) antibodyLAT2 适用: 人, 小鼠 WB 宿主: 小鼠 Monoclonal NAP-07 unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (Internal Region) antibodyVerified LAT2 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Western blotting: Recommended dilution: 1-2 μg/mL.
Flow cytometry: Intracellular staining, recommended dilution: 1-4 μg/mL, positive tissue: RAJI human lymphoma cell line, RAMOS human lymphoma cell line. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "The HDAC class I-specific inhibitor entinostat (MS-275) effectively relieves epigenetic silencing of the LAT2 gene mediated by AML1/ETO." in: Oncogene, Vol. 30, Issue 27, pp. 3062-72, (2011) (PubMed).
: "Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells." in: Journal of immunological methods, Vol. 328, Issue 1-2, pp. 139-51, (2007) (PubMed).
: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." in: Blood, Vol. 104, Issue 1, pp. 207-14, (2004) (PubMed).
: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, (2004) (PubMed).
: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).
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: "The HDAC class I-specific inhibitor entinostat (MS-275) effectively relieves epigenetic silencing of the LAT2 gene mediated by AML1/ETO." in: Oncogene, Vol. 30, Issue 27, pp. 3062-72, (2011) (PubMed).
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- LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))
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别名
- NTAL
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背景
- Linker for activation of T cells family member 2,NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.,LAB, LAT2
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基因ID
- 56743
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UniProt
- Q9JHL0
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途径
- Fc-epsilon Receptor Signaling Pathway, BCR Signaling
抗原
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