NEFH 抗体
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北京 101111
Quick Overview for NEFH 抗体 (ABIN94433)
抗原
See all NEFH 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Neurofilament H Purified
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特异性
- The antibody NF-01 recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa intracellular antigen) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
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交叉反应 （详细）
- Mammalian
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
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亚型
- IgG1
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anti-Neurofilament, Heavy Polypeptide (NEFH) antibody
NEFH 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) antibodyNEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal NF421 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 2-251) antibodyNEFH 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2C12F3 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 2-251) antibodyNEFH 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 4F2G12 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 1-100) antibodyNEFH 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) antibodyNEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig, Cow, 兔, 豚鼠, Gerbil, Cat WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal NE14 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 968-1020) antibodyNEFH 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 8H10 unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 968-1020) antibodyNEFH 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 21-120) antibodyNEFH 适用: 人, 大鼠, 小鸡 WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 821-1020) antibodyNEFH 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/mL.
Western blotting: Recommended dilution: 1-2 μg/mL. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord." in: Histochemistry, Vol. 100, Issue 6, pp. 495-502, (1994) (PubMed).
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: "Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord." in: Histochemistry, Vol. 100, Issue 6, pp. 495-502, (1994) (PubMed).
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- NEFH (Neurofilament, Heavy Polypeptide (NEFH))
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别名
- Neurofilament H
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背景
- Neurofilament heavy,Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights –, neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 amino acids) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic amino acids. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Plasma neurofilament heavy chain level has been proposed as a marker of axonal injury and clinical use of its degeneration and loss has been suggested as a biomarker of several neurodegenerative diseases.,NFH, NEFH, Neurofilament heavy chain, CMT2CC
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基因ID
- 4744
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UniProt
- P12036
抗原
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