LIME 抗体 (AA 281-296)
(2 references)Quick Overview for LIME 抗体 (AA 281-296) (ABIN94426)
抗原
See all LIME (LIME1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 281-296
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原理
- Anti-LIME1 Purified
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特异性
- The antibody LIME-10 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
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交叉反应 （详细）
- Human
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin.
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亚型
- IgG2a
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anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 141-295) antibody
LIME1 适用: 人 IP, ICFC 宿主: 小鼠 Monoclonal LIME-06 unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 170-295) antibodyLIME1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 141-295) antibodyLIME1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (C-Term) antibodyVerified LIME1 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 86-202) antibodyLIME1 适用: 小鼠 WB, IP 宿主: 小鼠 Monoclonal mLIME-05 unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 1-295) antibodyLIME1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL.
Western blotting: Reducing conditions, recommended dilution: 1 μg/mL.
Flow cytometry: Recommended dilution: 1-5 μg/mL. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, (2005) (PubMed).
: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).
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: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, (2005) (PubMed).
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- LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))
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别名
- LIME1
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背景
- Lck interacting transmembrane adaptor 1,LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.,LITA1
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基因ID
- 54923
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UniProt
- Q9H400
抗原
-
