LIME 抗体 (AA 141-295)
(1 reference)Quick Overview for LIME 抗体 (AA 141-295) (ABIN94424)
抗原
See all LIME (LIME1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 141-295
-
原理
- Anti-LIME1 Purified
-
特异性
- The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
-
交叉反应 （详细）
- Human
-
纯化方法
- Purified by protein-A affinity chromatography.
-
纯度
- > 95 % (by SDS-PAGE)
-
免疫原
- Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
-
亚型
- IgG1 kappa
-
-
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 170-295) antibody
LIME1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 141-295) antibodyLIME1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 281-296) antibodyLIME1 适用: 人 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal LIME-10 unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 86-202) antibodyLIME1 适用: 小鼠 WB, IP 宿主: 小鼠 Monoclonal mLIME-05 unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (C-Term) antibodyVerified LIME1 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 1-295) antibodyLIME1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Flow cytometry: Recommended dilution: 8-12 μg/mL. Intracellular staining.
-
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Do not freeze.
-
储存条件
- 4 °C
-
储存方法
- Store at 2-8°C. Do not freeze.
-
-
-
: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).
-
: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003) (PubMed).
-
- LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))
-
别名
- LIME1
-
背景
- Lck interacting transmembrane adaptor 1,LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.,LITA1
-
基因ID
- 54923
-
UniProt
- Q9H400
抗原
-
