LIME 抗体 (AA 141-295)
Quick Overview for LIME 抗体 (AA 141-295) (ABIN94424)
抗原
See all LIME (LIME1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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                                            抗原表位
- AA 141-295
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                                            原理
- Anti-LIME1 Purified
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                                            特异性
- The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
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                                            交叉反应 (详细)
- Human
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                                            纯化方法
- Purified by protein-A affinity chromatography.
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                                            纯度
- > 95 % (by SDS-PAGE)
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                                            免疫原
- Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
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                                            亚型
- IgG1 kappa
 
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                                            应用备注
- Flow cytometry: Recommended dilution: 8-12 μg/mL. Intracellular staining.
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                                            限制
- 仅限研究用
 
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                                            浓度
- 1 mg/mL
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                                            缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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                                            储存液
- Sodium azide
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                                            注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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                                            注意事项
- Do not freeze.
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                                            储存条件
- 4 °C
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                                            储存方法
- Store at 2-8°C. Do not freeze.
 
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                                            : "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling."    in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003)         (PubMed).        
 
 
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                                            : "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling."    in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, (2003)         (PubMed).        
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    - LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))
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                                            别名
- LIME1
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                                            背景
- Lck interacting transmembrane adaptor 1,LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.,LITA1
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                                            基因ID
- 54923
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                                            UniProt
- Q9H400
 抗原
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