(2 references)-
- 抗原 See all IgE 抗体
- IgE
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This IgE antibody is un-conjugated
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应用范围
- ELISA, Flow Cytometry (FACS)
- 原理
- Anti-Hu IgE Purified
- 特异性
- The mouse monoclonal antibody 4G7.325 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4H10 antibodies to IgE. The epitope is located within the amino acids 103-115 (WSDYNFDYSSSEE).
- 交叉反应 （详细）
- Human
- 纯化方法
- Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Purified human IgE.
- 克隆位点
- 4G7-325
- 亚型
- IgG
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anti-IgE antibody (APC)
适用: 人 FACS 宿主: 小鼠 Monoclonal 4H10 APC
anti-IgE antibody (PE)适用: 人 FACS 宿主: 小鼠 Monoclonal 4H10 PE
anti-IgE antibody (FITC)适用: 人 FACS 宿主: 小鼠 Monoclonal BE5 FITC
anti-IgE antibody (Biotin)适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal BE5 Biotin
anti-IgE antibody (PE)适用: 人 FACS 宿主: 小鼠 Monoclonal BE5 PE
anti-IgE (AA 1-421) antibody适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Flow cytrometry: Recommended dilution: 1-4 μg/mL.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "The molecular basis of antigenic cross-reactivity between the group 2 mite allergens." in: The Journal of allergy and clinical immunology, Vol. 107, Issue 6, pp. 977-84, (2001) (PubMed).
: "Hydrogen exchange nuclear magnetic resonance spectroscopy mapping of antibody epitopes on the house dust mite allergen Der p 2." in: The Journal of biological chemistry, Vol. 276, Issue 12, pp. 9359-65, (2001) (PubMed).
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: "The molecular basis of antigenic cross-reactivity between the group 2 mite allergens." in: The Journal of allergy and clinical immunology, Vol. 107, Issue 6, pp. 977-84, (2001) (PubMed).
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- 抗原
- IgE
- Abstract
- IgE 产品
- 背景
- Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E
- 分子量
- 180 kDa
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