HLA-E 抗体
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北京 101111
Quick Overview for HLA-E 抗体 (ABIN94356)
抗原
See all HLA-E 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-HLA-E Purified
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交叉反应 （详细）
- Human
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产品特性
- The antibody MEM-E/07 recognizes an extracellular epitope on native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
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亚型
- IgG1
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anti-HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E) (AA 251-358) antibody
HLA-E 适用: 人 WB, FACS, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E) antibodyHLA-E 适用: 人, Non-Human Primate FACS, IP, IHC (p) 宿主: 小鼠 Monoclonal MEM-E-06 unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E) (AA 1-358) antibodyHLA-E 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E) (AA 22-358) antibodyHLA-E 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E) antibody (Biotin)HLA-E 适用: 人 FACS, IP, IHC (p) 宿主: 小鼠 Monoclonal MEM-E-07 Biotin
anti-HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E) (AA 1-100) antibodyHLA-E 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E) antibodyHLA-E 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E) antibodyHLA-E 适用: 人, 灵长类 FACS, IP, IHC, IHC (p) 宿主: 小鼠 Monoclonal MEM-E-06 unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E) (AA 1-358) antibodyHLA-E 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E) antibodyHLA-E 适用: 人, 灵长类 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal MEM-E-02 unconjugated
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应用备注
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Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: spleen.
Flow cytometry: Recommended dilution: 1-4 μg/mL -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "MicroRNA editing facilitates immune elimination of HCMV infected cells." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003963, (2014) (PubMed).
: "Serum soluble HLA-E in melanoma: a new potential immune-related marker in cancer." in: PLoS ONE, Vol. 6, Issue 6, pp. e21118, (2011) (PubMed).
: "Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells." in: Neoplasia (New York, N.Y.), Vol. 13, Issue 9, pp. 822-30, (2011) (PubMed).
: "Glycodelin A suppresses the cytolytic activity of CD8+ T lymphocytes." in: Molecular immunology, Vol. 47, Issue 15, pp. 2458-66, (2010) (PubMed).
: "Lytic granule polarization, rather than degranulation, is the preferred target of inhibitory receptors in NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 8, pp. 4698-704, (2010) (PubMed).
: "HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines." in: Human immunology, Vol. 66, Issue 1, pp. 1-12, (2004) (PubMed).
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: "MicroRNA editing facilitates immune elimination of HCMV infected cells." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003963, (2014) (PubMed).
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- HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))
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别名
- HLA-E
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背景
- Major histocompatibility complex, class I, E,HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).,HLA class I histocompatibility antigen, alpha chain E, MHC class I antigen E
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基因ID
- 3133
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UniProt
- P13747
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
抗原
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