Keratin 7/17 抗体
(1 reference)Quick Overview for Keratin 7/17 抗体 (ABIN94296)
抗原
See all Keratin 7/17 (KRT7/17) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Cytokeratin 7/17 Purified
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特异性
- The antibody C-46 reacts with cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.
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无交叉反应
- 小鼠, 兔, 大鼠
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交叉反应 （详细）
- Porcine, Human, Bovine
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
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亚型
- IgG1
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anti-Keratin 7/17 (KRT7/17) antibody
KRT7/17 适用: 人, Cow IHC, WB, FACS, IF, StM 宿主: 小鼠 Monoclonal C-46 unconjugated
anti-Keratin 7/17 (KRT7/17) antibodyKRT7/17 适用: 人, Cow, Pig IHC, WB, IP, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, pretreatment: 0,1 % pepsin, 30 min, 37 °C, incubation 4 °C overnight, chromogen DAB, positive tissue: pancreas.
Western blotting: Recommended dilution: 1-2 μg/mL, positive control: HeLa cells. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis." in: Neoplasma, Vol. 37, Issue 3, pp. 333-42, (1990) (PubMed).
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: "A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis." in: Neoplasma, Vol. 37, Issue 3, pp. 333-42, (1990) (PubMed).
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- Keratin 7/17 (KRT7/17)
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别名
- Cytokeratin 7/17
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背景
- Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K7, K17, CK7, CD17, CK17, CYK7, CYK17, KRT7, KRT17, SCL, K2C7
抗原
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