Keratin 7/17 抗体
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- 抗原 See all Keratin 7/17 (KRT7/17) 抗体
- Keratin 7/17 (KRT7/17)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Keratin 7/17 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- It recognizes intermediate filament proteins (IFP's) of 55kDa and 46kDa, which are identified as cytokeratin 7 and Cytokeratin 17 respectively. On formalin-fixed, paraffin embedded sections, C-46 reacts with keratin 7 only. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Cytokeratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Cytokeratin 17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. Cytokeratin 17 antibody marks basal cell differentiation.
- 无交叉反应
- 雪貂, 小鼠, 兔, 大鼠
- 交叉反应 （详细）
- Human. Cow. Does not react with Mouse, Rat, Ferret or Rabbit.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Cytoskeletal preparation from HeLa cells (human cervical carcinoma cells)
- 克隆位点
- C-46
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product KRT7/17 Primary Antibody
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anti-Keratin 7/17 (KRT7/17) antibody
KRT7/17 适用: 人, Cow, Pig WB, IP, ICC, IHC (p) 宿主: 小鼠 Monoclonal C-46 unconjugated
anti-Keratin 7/17 (KRT7/17) antibodyKRT7/17 适用: 人, Cow WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal C-46 unconjugated
anti-Keratin 7/17 (KRT7/17) antibodyKRT7/17 适用: 人, Cow, Pig WB, IHC, IP, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or ME-180 cells. Skin or hepatocellular carcinoma.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- Keratin 7/17 (KRT7/17)
- 别名
- KRT7 & KRT17 (KRT7/17 产品)
- 别名
- KRT7 antibody, CK7 antibody, keratin 7 antibody, KRT7 antibody
- 背景
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CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7, CK17, K17, Keratin Type I Cytoskeletal 17, Keratin-17, KRT17, PCHC1,Cytokeratin 7/17
Cellular localisation: Cytoplasmic - 分子量
- 55kDa (CK7), 46kDa (CK17)
- 基因ID
- 3855, 3872, 411501
- UniProt
- P08729, Q04695
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