Keratin 7/17 抗体
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北京 101111
Quick Overview for Keratin 7/17 抗体 (ABIN7865681)
抗原
See all Keratin 7/17 (KRT7/17) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-Keratin 7+17 mouse monoclonal, C-46, purified
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交叉反应 （详细）
- not with ferret, mouse, rabbit, rat
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纯化方法
- affinity chromatography
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免疫原
- purified human keratin 7, isolated from HeLa cytoskeleton
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亚型
- IgG1 kappa
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anti-Keratin 7/17 (KRT7/17) antibody
KRT7/17 适用: 人, Cow, Pig WB, ICC, IP, IHC (p) 宿主: 小鼠 Monoclonal C-46 unconjugated
anti-Keratin 7/17 (KRT7/17) antibodyKRT7/17 适用: 人, Cow IHC, WB, FACS, IF 宿主: 小鼠 Monoclonal C-46 unconjugated
anti-Keratin 7/17 (KRT7/17) antibodyKRT7/17 适用: 人, Cow, Pig IHC, WB, IP, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)
FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment and/ or pepsin digestion recommended)
WB_dilution: 1:50-1:100 (1-2 μg/mL)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- store at 2 - 8 °C
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- Keratin 7/17 (KRT7/17)
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别名
- Keratin 7+17
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背景
- C-46 reacts with keratins 7 (54 kDa) and 17 (46 kDa). On formalin-fixed paraffin embedded sections C46 reacts with keratin 7 only. Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia. Positive control: Relevant simple epithelia like ovary, lung, and breast.
抗原
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