Cytokeratin 18 抗体
Our Local Distributor
北京 101111
Quick Overview for Cytokeratin 18 抗体 (ABIN94279)
抗原
See all Cytokeratin 18 (KRT18) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Anti-Cytokeratin 18 Purified
-
特异性
- The antibody C-04 reacts with cytokeratin 18 (45 kDa intracellular antigen), a member of intermediate filaments subfamily represented in epithelial tissues.
-
交叉反应 (详细)
- Mammalian
-
纯化方法
- Purified by protein-A affinity chromatography.
-
纯度
- > 95 % (by SDS-PAGE)
-
免疫原
- Cytoskeleton preparation of epidermal carcinoma cell line A431.
-
亚型
- IgG1
-
-
-
-
应用备注
-
Western blotting: Recommended dilution: 1-2 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive control: colon, kidney.
ELISA: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of cytokeratin 18 in combination with the antibody DA-7 (cat. no. 1B-110-C100) and with the antibody DC-10 (cat. no. 1B-107-C100).
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining. -
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Do not freeze.
-
储存条件
- 4 °C
-
储存方法
- Store at 2-8°C. Do not freeze.
-
-
-
: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).
: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, (1990) (PubMed).
: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, (1989) (PubMed).
: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, (1989) (PubMed).
-
: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).
-
- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
-
别名
- Cytokeratin 18
-
背景
- Keratin 18,Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).,K18, CK18, CYK18, KRT18
-
基因ID
- 3875
-
UniProt
- P05783
-
途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
-