CSK 抗体 (AA 330-450)
Quick Overview for CSK 抗体 (AA 330-450) (ABIN94268)
抗原
See all CSK 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 330-450
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原理
- Anti-CSK Purified
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特异性
- The mouse monoclonal antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa intracellular non-receptor protein tyrosine kinase.
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交叉反应 （详细）
- Human, Mouse, Other not determined
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450.
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亚型
- IgG1
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anti-C-Src tyrosine Kinase (CSK) (AA 1-100) antibody
CSK 适用: 人 WB, ELISA, IP, IHC (p), RNAi 宿主: 小鼠 Monoclonal 3A3 unconjugated
anti-C-Src tyrosine Kinase (CSK) (C-Term) antibodyVerified CSK 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-C-Src tyrosine Kinase (CSK) antibodyCSK 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-C-Src tyrosine Kinase (CSK) antibodyCSK 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 5F3 unconjugated
anti-C-Src tyrosine Kinase (CSK) (AA 243-438) antibodyCSK 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-C-Src tyrosine Kinase (CSK) (AA 325-416) antibodyCSK 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-C-Src tyrosine Kinase (CSK) (AA 67-101), (N-Term) antibodyCSK 适用: 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB50568 unconjugated
anti-C-Src tyrosine Kinase (CSK) (Ser280) antibodyCSK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-C-Src tyrosine Kinase (CSK) (AA 241-450) antibodyCSK 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-C-Src tyrosine Kinase (CSK) (AA 1-200) antibodyCSK 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line.
Western blotting: The recommended dilution of the antibody CSK-04 is 1:250-1:300, weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions.
Immunoprecipitation: The immunosorbent (CSK-04 antibody covalently bound to beads) immunoprecipitates Csk from cell lysates. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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- CSK (C-Src tyrosine Kinase (CSK))
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别名
- CSK
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背景
- C-terminal Src kinase,C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them it lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases, that are localized in lipid rafts, is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.
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基因ID
- 1445
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UniProt
- P41240
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途径
- TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events
抗原
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