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ICAM1 抗体 (Biotin)

This anti-ICAM1 antibody is a 小鼠 单克隆 antibody detecting ICAM1 in WB, FACS, ICC, IHC (p) 和 IHC (fro). Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN94179
发货至: 中国

Quick Overview for ICAM1 抗体 (Biotin) (ABIN94179)

抗原

See all ICAM1 抗体
ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

适用

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小鼠

克隆类型

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单克隆

标记

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This ICAM1 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

1H4
  • 原理

    Anti-Hu CD54 Biotin

    特异性

    The antibody 1H4 recognizes an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells, the expression of CD54 is upregulated by activation.

    交叉反应 (详细)

    Other not tested, Human

    纯化方法

    Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

    免疫原

    Raji cells and spleen cells fused with NS1 cells

    亚型

    IgG2b
  • 应用备注

    Flow cytometry: Recommended dilution: 2-5 μg/mL.

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Do not freeze.
    Avoid prolonged exposure to light.

    储存条件

    4 °C

    储存方法

    Store at 2-8°C. Do not freeze.
  • Williams, Chaudhry, Goodfellow, Lea, Evans: "Interactions of decay-accelerating factor (DAF) with haemagglutinating human enteroviruses: utilizing variation in primate DAF to map virus binding sites." in: The Journal of general virology, Vol. 85, Issue Pt 3, pp. 731-8, (2004) (PubMed).

  • 抗原

    ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

    别名

    CD54

    背景

    Intercellular adhesion molecule 1,CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.,ICAM-1, BB2, P3.58

    基因ID

    3383

    UniProt

    P05362

    途径

    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
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