CD44 抗体
(2 references)Quick Overview for CD44 抗体 (ABIN94121)
抗原
See all CD44 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu CD44 Purified
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特异性
- The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.), it is negative on platelets and hepatocytes.
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交叉反应 （详细）
- Human, Porcine, Canine (Dog)
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- COS-7 cells (African Green Monkey).
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亚型
- IgG1
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anti-CD44 (CD44) antibody
CD44 适用: 人, 小鼠 FACS, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8E2F3 unconjugated
anti-CD44 (CD44) antibodyKO Validated CD44 适用: 人 WB, IF, IHC (p), IP, IHC (fro), ICC, LCI 宿主: 兔 Polyclonal unconjugated
anti-CD44 (CD44) (AA 21-220) antibodyCD44 适用: 人 FACS, WB, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 6G2D2 unconjugated
anti-CD44 (CD44) antibodyCD44 适用: 人 FACS, WB, IHC, ELISA, IHC (p), IP, Inhibition 宿主: 小鼠 Monoclonal MEM-85 unconjugated
anti-CD44 (CD44) (AA 148-247) antibodyCD44 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD44 (CD44) (AA 36-194) antibodyCD44 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7F4F1 unconjugated
anti-CD44 (CD44) (AA 21-259) antibodyCD44 适用: 人, 小鼠, 大鼠 FACS, WB, IHC, ELISA, IF, IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-CD44 (CD44) (AA 1-361) antibodyCD44 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-CD44 (CD44) antibodyCD44 适用: 人, Baboon, Green Monkey FACS, WB, IHC, IF, Func, StM 宿主: 小鼠 Monoclonal 156-3C11 unconjugated
anti-CD44 (CD44) (AA 35-300) antibodyCD44 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow cytometry: Recommended dilution: 4 μg/mL.
Western blotting: Recommended dilution: 2 μg/mL, 60 min on vertical incubator, positive control: Kg-1a human acute leukemia cell lysate, JURKAT human leukemia T-cell lysate, non-reducing conditions, 6 % separating gel.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: uterus, myometrium. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Characterisation of stemness and multipotency of ovine muscle-derived stem cells from various muscle sources." in: Journal of anatomy, Vol. 239, Issue 2, pp. 336-350, (2021) (PubMed).
: "Vascular wall-resident CD44+ multipotent stem cells give rise to pericytes and smooth muscle cells and contribute to new vessel maturation." in: PLoS ONE, Vol. 6, Issue 5, pp. e20540, (2011) (PubMed).
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: "Characterisation of stemness and multipotency of ovine muscle-derived stem cells from various muscle sources." in: Journal of anatomy, Vol. 239, Issue 2, pp. 336-350, (2021) (PubMed).
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- CD44
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别名
- CD44
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背景
- CD44 Molecule,CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.,PGP-I, HUTCH-I, ECMR-III, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, MC56, MIC4, INLU, LHR
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基因ID
- 960
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UniProt
- P16070
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途径
- Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
抗原
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