CD34 抗体
(1 reference)Quick Overview for CD34 抗体 (ABIN94077)
抗原
See all CD34 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu CD34 Purified
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特异性
- The mouse monoclonal antibody 4H11[APG] reacts with extracellular class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
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交叉反应 （详细）
- Human
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
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亚型
- IgG1
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anti-CD34 (CD34) (AA 366-382), (C-Term) antibody
CD34 适用: 人, 大鼠, 小鼠 FACS, WB, IHC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 201-300) antibodyCD34 适用: 人, 大鼠, 小鼠, 兔 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 301-385) antibodyCD34 适用: 人, 大鼠, 小鼠, 兔, 小鸡, 山羊 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 36-287) antibodyCD34 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) antibodyCD34 适用: 人, 大鼠 FACS, IHC, StM 宿主: 兔 Monoclonal HPCA1-2598R unconjugated Recombinant Antibody
anti-CD34 (CD34) antibodyCD34 适用: 人, Non-Human Primate FACS, WB, IHC (p), IP, IHC (fro), Func 宿主: 小鼠 Monoclonal QBEnd-10 unconjugated
anti-CD34 (CD34) antibody (PE)CD34 适用: 人 FACS, ICC 宿主: 小鼠 Monoclonal 4H11[APG] PE
anti-CD34 (CD34) (C-Term) antibodyCD34 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 151-385) antibodyCD34 适用: 人, 大鼠 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 124-375) antibodyCD34 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blotting: Recommended dilution: 1-2 μg/mL, positive control: Kg-1a, TF-1 cells, non-reducing conditions.
Flow cytometry: Recommended dilution: 2 μg/mL.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: placenta endothelium. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines." in: Neoplasma, Vol. 54, Issue 4, pp. 311-20, (2007) (PubMed).
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: "Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines." in: Neoplasma, Vol. 54, Issue 4, pp. 311-20, (2007) (PubMed).
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- CD34
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别名
- CD34
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背景
- CD34 Molecule,CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
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基因ID
- 947
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UniProt
- P28906
抗原
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