D1 Protein of PSII, (PsbA) (C-Term) 抗体
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北京 101111
Quick Overview for D1 Protein of PSII, (PsbA) (C-Term) 抗体 (ABIN93523)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Anti-PsbA | D1 protein of PSII, C-terminal (chicken)
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特异性
- The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthasesubcomplex, as well as the chloroplastic CF1 ATP synthase and most knownbacterial F-type ATP synthases. Peptide used for antibody product.
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交叉反应 （详细）
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Alaria esculenta, Amphidinium carterae, Anabaena sp., Arabidopsis thaliana, Brachypodium sylvaticum, Chlamydomonas reinhardtii, Chlamydomonas raudensis (both Antarctic and mesophilic strains), Cyanophora sp. , Cyanothece sp. ATCC 51142, Cynara cardunculus, Gonyaulax polyedra, Fucus vesiculosus, Horderum vulgare, Lobaria pulmonaria, Petunia sp., Pinus sylvestris, Spartina alterniflora, Solanum lycopresicum, Synechococcus sp. PCC 7942, Triticum aestivum, Ulva sp., symbiotic dinoflagellates of Stylophora pistillata and Turbinaria reniformis, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Algae (brown,red),Conifers,Cryptomonads,Legumes,Stramenopiles,Euglenoids,Prochlorophytes,Xantophytes
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纯化方法
- Purified, total IgY
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免疫原
- KLH-conjugated synthetic peptide derived from available plant
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亚型
- IgY
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anti-D1 Protein of PSII, (PsbA) (C-Term) antibody
适用: Arabidopsis thaliana, Chlamydomonas reinhardtii, Zea mays, Synechococcus sp., Barley, Anabaena variabilis, Triticum aestivum, Tomato, Spartina alterniflora, Chlorella vulgaris, Synechocystis, Quercus ilex, Miscanthus giganteus, Nicotiana benthamiana, Nicotiana tabacum, Paulinella chromatophora, Pea, Phaeodactylum tricornutum, Physcomytrium Patens, Picea glauca, Pilosella officinarum, Pinus strobus, Prochlorococcus, Proso millet (Panicum miliaceum), Mirkania micrantha, Red Algae (Cyanidioschyzon), Salicornia bigelovii, Soybean, Spinach, Spirodela polyrhiza, Symbiodinium, Alniaria alnifolia, Syntrichia ruralis, Tetradesmus obliquus, Thalassiosira weisflogii, Triticosecale, Alfalfa, Dianthus caryophyllus, Artemisia annua, Begonia, Cannabis sativa L., Chlorella ohadii, Araliaceae (Panax), Chromera velia, Cladocopium goreaui, Colobanthus quitensis, Coscinodiscus wailesii, Craterostigma, Cytisus cantabricus, Desmodesmus, Arundo, Diatom (Skeletonema), Ditylum brightwellii, Eucalyptus globulus, Fraxinus chinensis subsp. Rhynchophylla, Gongolaria barbata, Halomicronema hongdechloris, Lasallia hispanica, Lindernia, Manihot esculenta, Marchantia polymorpha, Megathyrsus maximus, Microcystis aeruginosa WB, BN PAGE, EM, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1 :4000-1 : 8000, 5 μg of total protein, (WB)
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说明
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The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions.In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.This antibody will also detect the phosphorylated form of D1as an alternate band to the main band on a high resolution gel.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- 4 °C
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储存方法
- Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Isolation and purification assay of ex vivo photosystem II D1 protein toward integrated biointeraction analysis." in: Analytical and bioanalytical chemistry, Vol. 390, Issue 4, pp. 1195-202, (2008) (PubMed).
: "Function and evolution of the psbA gene family in marine Synechococcus: Synechococcus sp. WH7803 as a case study." in: The ISME journal, Vol. 2, Issue 9, pp. 937-53, (2008) (PubMed).
: "Ammonia triggers photodamage of photosystem II in the cyanobacterium Synechocystis sp. strain PCC 6803." in: Plant physiology, Vol. 147, Issue 1, pp. 206-15, (2008) (PubMed).
: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." in: The New phytologist, Vol. 177, Issue 2, pp. 428-42, (2008) (PubMed).
: "Iron limitation in the marine cyanobacterium Trichodesmium reveals new insights into regulation of photosynthesis and nitrogen fixation." in: The New phytologist, Vol. 179, Issue 3, pp. 784-98, (2008) (PubMed).
: "Heat stress causes inhibition of the de novo synthesis of antenna proteins and photobleaching in cultured Symbiodinium." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 11, pp. 4203-8, (2008) (PubMed).
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: "Isolation and purification assay of ex vivo photosystem II D1 protein toward integrated biointeraction analysis." in: Analytical and bioanalytical chemistry, Vol. 390, Issue 4, pp. 1195-202, (2008) (PubMed).
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- D1 Protein of PSII, (PsbA)
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背景
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Background: The PsbA (D1) protein of Photosystem II is rapidly cycled under illumination in all oxygenic photobionts. Disruption of PsbA cycling or losses of PsbA pools are central to photoinhibition of photosynthesis in cyanobacteria, algae and plants under a wide range of conditions including excess light, low temperature and UV exposure. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.
Additional information: A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24 kDa and 16 kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands. Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.Example of a simulataneous western blot detection with RbcL, PsbA and PsaC antibodies.
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分子量
- 38 | 28-30 kDa
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基因ID
- 844802
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UniProt
- P83755, P0C434, Q14FH6, P14660
抗原
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