D1 Protein of PSII, (PsbA) (C-Term) 抗体
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北京 101111
Quick Overview for D1 Protein of PSII, (PsbA) (C-Term) 抗体 (ABIN2559394)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Anti-PsbA | D1 protein of PSII, C-terminal (rabbit antibody) (thylakoid membrane marker)
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交叉反应 （详细）
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Alniaria alnifolia, Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp., Begonia sp. , Cannabis sativa L., Cladocopium goreaui, Chlamydomonas reinhardtii, Chlorella ohadii, Chromera velia, Chlorella vulgaris, Colobanthus quitensis (Kunth) Bartl, Coscinodiscus wailesii, Craterostigma sp., Cyanidioschyzon merolae, Cytisus cantabricus (Wilk.) Rchb. F, Desmodesmus sp., Dianthus caryophyllus, Ditylum brightwellii, Eucalyptus globulus, Fraxinus rhynchophylla, Glycine max, Gongolaria barbata,Halomicronema hongdechloris, Hieracium pilosella L., Hordeum vulgare, Lasallia hispanica, Lindernia sp., Manihot esculenta, Marchantia polymorpha (liverwort), Medicago truncatula, Miscanthus x giganteus, Microcystis aeruginosa, Mirkania micrantha, Nicotiana benthamiana, Nicotiana tabcum, Panicum miliaceum, Panax ginseng, Panicum maximum, Paulinella chromatophora (amoeba), Pheodactylum tricornutum CCAP 1055/1, Physcomitrium patens, Picea glauca, Pinus strobus, Pisum sativum, Prochlorococcus sp. (surface and deep water ecotype), Salicornia bigelovii , Skeletonema costatum (diatom), Solanum lycopersicum, Spartina alterniflora, Spinacia oleracea, Spirodela polyrhiza, Symbiodinium sp, Synechococcus sp. PCC 7942, Synechococcus elongatus UTEX 2973, Synechocystis sp. 6803, Syntrichia muralis, Thalassiosira weissflogii, Tetradesmus obliquus, Triticum aestivum, Triticale, Zea may, Quercus ilex
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Algae (brown,red),Brassica napus,Carica papaya,Conifers,Cyanobacteria,Cannabis sativa,Citrus sinensis,Dicots,Eragrostis tef,Galdieria sulphuraria,Lactuca sativa,Lycopersicum esculentum,Medicago sativa,Nannochloropsis sp.,Oryza sativa,Ostreococcus sp.,Phalaenopsis aphrodite,Pisum sativum,Porphyridium purpureum,Sesamum indicum,Thalassiosira pseudonana,Zosteria marina,Vitis vinifera cellular [compartment marker] of thylakoid membrane
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纯化方法
- Serum
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免疫原
- KLH-conjugated synthetic peptide derived from available plant
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anti-D1 Protein of PSII, (PsbA) (C-Term) antibody
适用: Arabidopsis thaliana, Chlamydomonas reinhardtii, Zea mays, Synechococcus sp., Anabaena variabilis, Triticum aestivum, Tomato, Spartina alterniflora, Petunia hybrida, Alaria esculenta, Ulva, Stylophora pistillata, Stony Coral (Turbinaria), Pinus sylvestris, Lobaria pulmonaria, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Gonyaulax polyedra, Fucus vesiculosus, Cynara cardunculus, Cyanothece, Cyanophora paradoxa, Chlamydomonas raudensis, Brachypodium sylvaticum, Amphidinium carterae WB 宿主: 小鸡 Polyclonal unconjugated
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应用备注
- 1: 500 (IF), 1: 200 (IG), 1 : 10 000 (WB)
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说明
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The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions. In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e.g. precursor and mature protein as compare to the hen anti-PsbA antibodies AS01 016.This antibody will detect the phosphorylated form of D1as an alternate band to the main band on a high resolution gel.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Modulations of physiological responses and possible involvement of defense-related secondary metabolites in acclimation of Artemisia annua L. against short-term UV-B radiation." in: Planta, Vol. 240, Issue 3, pp. 611-27, (2014) (PubMed).
: "A temporal regulatory mechanism controls the different contribution of endoplasmic reticulum and plastidial ω-3 desaturases to trienoic fatty acid content during leaf development in soybean (Glycine max cv Volania)." in: Phytochemistry, Vol. 95, pp. 158-67, (2013) (PubMed).
: "Trafficking of protein into the recently established photosynthetic organelles of Paulinella chromatophora." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 14, pp. 5340-5, (2012) (PubMed).
: "Composition and physiological function of the chloroplast NADH dehydrogenase-like complex in Marchantia polymorpha." in: The Plant journal : for cell and molecular biology, Vol. 72, Issue 4, pp. 683-93, (2012) (PubMed).
: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, (2011) (PubMed).
: "The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism." in: BMC plant biology, Vol. 11, pp. 108, (2011) (PubMed).
: "Superoxide anion radicals generated by methylviologen in photosystem I damage photosystem II." in: Physiologia plantarum, (2010) (PubMed).
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- D1 Protein of PSII, (PsbA)
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背景
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Background: The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1
Additional information: Due to biology of PsbA (D1) protein a number of degradation products can apprear in a sample and may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24 kDa and 16 kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands.Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.This product can be sold containing ProClin if requested.
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分子量
- 38 | 28-30 kDa
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基因ID
- 844802
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UniProt
- P83755, P0C434, Q14FH6, Q6YXN7, P07753, P14660
抗原
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