KLH 抗体
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北京 101111
Quick Overview for KLH 抗体 (ABIN93503)
抗原
See all KLH 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-KLH | Keyhole limpet hemocyanin
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交叉反应 （详细）
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Megathura crenulata - most commonly used carrier protein
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
纯化方法
- Immunogen affinity purified serum
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免疫原
- Purified keyhole limpet hemocyanin (KLH)
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亚型
- IgG
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anti-Keyhole Limpet Hemocyanin (KLH) antibody
KLH 适用: Keyhole Limpet ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1: 10 000 (ELISA), 1: 10 000 (WB), 1: 1000 (IL)
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说明
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Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- KLH (Keyhole Limpet Hemocyanin (KLH))
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背景
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Background: Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host's immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.
Additional information: Protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide.
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分子量
- ca. 400 kDa/subunit
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UniProt
- Q6KC56
抗原
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