KLH 抗体
This anti-KLH antibody is a 兔 多克隆 antibody detecting KLH in ELISA, WB 和 IL. Suitable for Keyhole Limpet.
Quick Overview for KLH 抗体 (ABIN93503)
抗原
See all KLH 抗体
KLH
(Keyhole Limpet Hemocyanin (KLH))
适用
Keyhole Limpet
宿主
兔
克隆类型
多克隆
标记
This KLH antibody is un-conjugated
应用范围
ELISA, Western Blotting (WB), Immunolocalization (IL)
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预测反应
- Megathura crenulata - most commonly used carrier protein
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产品特性
- Expected / apparent Molecular Weight of the Antigene: Aprox. 400 kDa
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纯化方法
- serum
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免疫原
- purified keyhole limpet hemocyanin (KLH), whole molecule
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亚型
- IgG
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anti-Keyhole Limpet Hemocyanin (KLH) antibody
KLH 适用: Keyhole Limpet ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Recommended Dilution: 1 : 10 000 (ELISA), 1 : 10 000 (WB), 1 : 1000 (IL).
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说明
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Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution please add 50 µL of dest. water
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缓冲液
- PBS pH 7.4
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注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
储存条件
- -20 °C
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- KLH (Keyhole Limpet Hemocyanin (KLH))
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背景
- Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host's immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.
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分子量
- ca. 400 kDa/subunit
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UniProt
- Q6KC56
抗原
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