KLH 抗体
-
- 抗原 See all KLH 抗体
- KLH (Keyhole Limpet Hemocyanin (KLH))
-
适用
- Keyhole Limpet
-
宿主
- 小鼠
-
克隆类型
- 多克隆
-
标记
- This KLH antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH protein
- 亚型
- IgG
- Top Product
- Discover our top product KLH Primary Antibody
-
anti-Keyhole Limpet Hemocyanin (KLH) antibody
KLH 适用: Keyhole Limpet ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Keyhole Limpet Hemocyanin (KLH) antibody (FITC)KLH 适用: Keyhole Limpet ELISA, WB, FM 宿主: 兔 Polyclonal FITC
anti-Keyhole Limpet Hemocyanin (KLH) antibody (HRP)KLH 适用: Keyhole Limpet ELISA, WB 宿主: 兔 Polyclonal HRP
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Rhodamine)KLH 适用: Keyhole Limpet ELISA, WB, FM 宿主: 兔 Polyclonal Rhodamine
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Alkaline Phosphatase (AP))KLH 适用: Keyhole Limpet ELISA 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Biotin)KLH 适用: Keyhole Limpet ELISA, WB 宿主: 兔 Polyclonal Biotin
anti-Keyhole Limpet Hemocyanin (KLH) antibody (Texas Red (TR))KLH 适用: Keyhole Limpet ELISA, WB, FM 宿主: 兔 Polyclonal Texas Red (TR)
anti-Keyhole Limpet Hemocyanin (KLH) antibodyKLH 适用: Keyhole Limpet ELISA, WB, IL 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200 - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C
- 有效期
- 12 months
-
- 抗原
- KLH (Keyhole Limpet Hemocyanin (KLH))
- Abstract
- KLH 产品
- 背景
-
Keyhole limpet hemocyanin is an extremely large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000 in aggregates with molecular weights of 4,500,000-13,000,000. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata.Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of for research, biotechnology and therapeutic applications. Haptens are substances with a low molecular weight such as peptides, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production.[2] KLH is the most widely employed carrier proteins for this purpose. KLH is an effective carrier protein for several reasons. Its large size and numerous epitopes generate a substantial immune response, and abundance of lysine residues for coupling haptens, allows a high hapten:carrier protein ratio increasing the likelihood of generating hapten-specific . In addition, because KLH is derived from the limpet, a gastropod, it is phylogenetically distant from mammalian proteins, thus reducing false positives in immunologically based research techniques in mammalian model organisms.KLH may also be a challenging molecule to work with because of its propensity to aggregate and precipitate. Aggregates remain immunogenic, but limit the ability to conjugate haptens and are difficult to manipulate in the laboratory.
Synonyms: Keyhole limpet hemocyanin KLH
-
