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- 抗原 See all DYKDDDDK Tag products
- DYKDDDDK Tag
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This DYKDDDDK Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)
- 序列
- DYKDDDD
- 纯化方法
- Protein A affinity chromatography
- 免疫原
- FLAG antibody was raised in mouse using DYKDDDDK (FLAG) synthetic peptide conjugated to KLH as the immunogen.
- 克隆位点
- FG4R
- 亚型
- IgG2b
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anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, IP, IF 宿主: 小鼠 Monoclonal 2C5 unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 4G5C8 unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated
anti-DYKDDDDK Tag antibodyD 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
anti-DYKDDDDK Tag antibody (HRP)WB, ELISA, IP 宿主: 兔 Polyclonal HRP
anti-DYKDDDDK Tag antibodyIP 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated
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- 应用备注
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WB: 1:1000-3000
Optimal conditions should be determined by the investigator. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.
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- 抗原
- DYKDDDDK Tag
- 别名
- DYKDDDD-Tag (DYKDDDDK Tag 产品)
- 物质类
- Tag
- 背景
- DYKDDDDK tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A DYKDDDDK-Tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a DYKDDDDK-Tag to this protein allows one to follow the protein with an antibody against the DYKDDDDK sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Synonyms: Monoclonal DYKDDDDK antibody, Anti DYKDDDDK antibody, DYKDDDDK antibody, DYKDDDDK antibody, FG4R antibody, DYKDDDDK tag antibody.
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