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Recombinant DYKDDDDK Tag 抗体 (Cleavage Site)

The 兔 单克隆 anti-DYKDDDDK Tag antibody is suitable to detect DYKDDDDK Tag. It has been validated for WB, ELISA, IP 和 ICC.
产品编号 ABIN7453373
发货至: 中国
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Our Local Distributor

中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant DYKDDDDK Tag 抗体 (Cleavage Site) (ABIN7453373)

抗原

DYKDDDDK Tag

抗体类型

Recombinant Antibody

宿主

  • 90
  • 87
  • 9
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  • 6

克隆类型

  • 103
  • 96
单克隆

标记

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This DYKDDDDK Tag antibody is un-conjugated

应用范围

  • 129
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Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

克隆位点

BLRE00G
  • 抗原表位

    • 6
    • 2
    • 1
    • 1
    Cleavage Site

    原理

    Rabbit anti-ECS (DYKDDDDK) Tag Recombinant Monoclonal Antibody [BLRE00G]

    纯化方法

    Purified

    免疫原

    Enterokinase Cleavage Site (DYKDDDDK)

    亚型

    IgG
  • 应用备注

    IP: 20 μL/mg lysate

    ICC: 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

    WB: 17:40:00

    ELISA: 1:1,000 - 1:75,000, for coating plates 1:100 - 1:500

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    Phosphate Buffered Saline (PBS) with 0.1 % BSA and 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    2 - 8 °C

    有效期

    12 months
  • 抗原

    DYKDDDDK Tag

    别名

    ECS (DYKDDDDK)

    物质类

    Tag

    背景

    The entereokinase cleavage site (ECS) tag is an epitope tag with the sequence DYKDDDDK. Its hydrophobic nature helps to maintain the structural integrity of the protein to which it is appended. Further, its nature as a kinase cleavage site allows for its removal from the recombinant protein if needed.
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