His Tag 抗体
Quick Overview for His Tag 抗体 (ABIN920801)
抗原
See all His Tag 抗体适用
宿主
克隆类型
标记
应用范围
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序列
- HHHHHH
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纯化方法
- Affinity purified
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anti-His Tag antibody
WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated
anti-His Tag antibody (Cy3)WB, ELISA, FACS, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 Cy3
anti-His Tag antibody (DyLight 800)WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 800
anti-His Tag antibody (DyLight 649)WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649
anti-His Tag antibody (HRP)WB, ELISA, IHC 宿主: 小鼠 Monoclonal 33D10-D2-G8 HRP
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应用备注
- Western blot: Use at 1:1,000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS pH 7.2 and 0.1% sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- His Tag
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别名
- Hexahistidine-Tag
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物质类
- Tag
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背景
- Chickens were immunized with HHHHHH peptide conjugated to KLH. After multiple immunizations in Freund's adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid support. Specification: Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH- BSA). A 1:8,000 dilution of the antibody gave an OD = 1.0 in a 15 minute reaction using HRP- conjugated goat anti-chicken IgY (IgG) at 1:10,000 at TMB as the substrate. Appropriate specificity controls were run.
抗原
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