ELISA: Use at 1:10,000. Western blot: Use at 1:2,000
限制
仅限研究用
状态
Liquid
缓冲液
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2% BSA and 0.1% thimerosal.
储存液
Thimerosal (Merthiolate)
注意事项
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
抗原
c-MYC (MYC)
(Myc Proto-Oncogene protein (MYC))
别名
C-Myc
背景
Chickens were immunized with the C-Myc peptide conjugated with KLH. After multiple immunizations in Freund's adjuvant, eggs were collected. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid phase. The antibodies were conjugated with horseradish peroxidase (HRP). Specification: ELISA: Antibody specificity was verified by ELISA against the peptide conjugated to BSA. A 1:20,000 dilution of the antibody gave an OD = 1.0 in a 15 minute reaction using TMB as the substrate. Appropriate specificity controls were run.