Small Basement Membrane Heparan Sulfate Proteoglycan 抗体
适用: 人
WB, IHC, ELISA
宿主: 小鼠
Monoclonal
1F10-B8
unconjugated
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- 抗原
- Small Basement Membrane Heparan Sulfate Proteoglycan
- 适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- 特异性
- Monoclonal Ab 1F10/B8 is specific for a core protein epitope of a human small basement membrane heparan sulfate proteoglycan (HS-PG). MAb 1F10/B8 recognizes an epitope different from that recognized by mAb 2H7/G11 and mAb 2E2/B5. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that at least two other basement membrane HS-PG exist: Agrin, originally discovered as an important component of the neuromuscular junction, and a novel small HS-PG which was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200 kDa (aorta) and 30-160 kDa (kidney) and a core protein size of 24 kDa or 22 kDa, respectively, was localized by immunohistochemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.
- 纯化方法
- Purified human small basement membrane heparan sulfate proteoglycan (HS-PG). Protein G purified.
- 克隆位点
- 1F10-B8
- 亚型
- IgG1 kappa
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- 应用备注
- ELISA (less than 1µg/ml), Western Blot (1µg/ml), immunohistochemistry (1µg/ml). This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Resuspend in aqua bidest.
- 缓冲液
- 50 mM TRIS pH 7.4
- 储存条件
- 4 °C
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- 抗原
- Small Basement Membrane Heparan Sulfate Proteoglycan
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