Small Basement Membrane Heparan Sulfate Proteoglycan 抗体
The 小鼠 单克隆 anti-Small Basement Membrane Heparan Sulfate Proteoglycan antibody is suitable to detect Small Basement Membrane Heparan Sulfate Proteoglycan in samples from 人. It has been validated for WB, IHC 和 ELISA.
产品编号 ABIN870825
发货至:
中国
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中国
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北京 101111
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Quick Overview for Small Basement Membrane Heparan Sulfate Proteoglycan 抗体 (ABIN870825)
抗原
Small Basement Membrane Heparan Sulfate Proteoglycan
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Western Blotting (WB), Immunohistochemistry (IHC), ELISA
克隆位点
1F10-B8
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特异性
- Monoclonal Ab 1F10/B8 is specific for a core protein epitope of a human small basement membrane heparan sulfate proteoglycan (HS-PG). MAb 1F10/B8 recognizes an epitope different from that recognized by mAb 2H7/G11 and mAb 2E2/B5. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that at least two other basement membrane HS-PG exist: Agrin, originally discovered as an important component of the neuromuscular junction, and a novel small HS-PG which was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200 kDa (aorta) and 30-160 kDa (kidney) and a core protein size of 24 kDa or 22 kDa, respectively, was localized by immunohistochemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.
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纯化方法
- Purified human small basement membrane heparan sulfate proteoglycan (HS-PG). Protein G purified.
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亚型
- IgG1 kappa
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应用备注
- ELISA (less than 1µg/ml), Western Blot (1µg/ml), immunohistochemistry (1µg/ml). This antibody has not been tested for use in all applications. This does not necessarily exclude its use for non-tested procedures. The stated dilutions are recommendations only.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Resuspend in aqua bidest.
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缓冲液
- 50 mM TRIS pH 7.4
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储存条件
- 4 °C
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- Small Basement Membrane Heparan Sulfate Proteoglycan
抗原
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