Small Basement Membrane Heparan Sulfate Proteoglycan 抗体
The 小鼠 单克隆 anti-Small Basement Membrane Heparan Sulfate Proteoglycan antibody (Clone 1F10-B8) (ABIN5540680) specifically detects Small Basement Membrane Heparan Sulfate Proteoglycan in WB, EIA 和 IHC (fro).
The antibody is reactive with 人 samples.
产品编号 ABIN5540680
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Small Basement Membrane Heparan Sulfate Proteoglycan 抗体 (ABIN5540680)
抗原
Small Basement Membrane Heparan Sulfate Proteoglycan
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
克隆位点
1F10-B8
-
-
特异性
- This Monoclonal antibody 1F10/B8 is specific for a core protein epitope of a Human small basement membrane Heparan Sulfate proteo-glycan (HS-PG). AM02102PU recognizes an epitope different from that recognized by clones 2H7/G11 (Cat.-No AM02103PU) and 2E2/B5 (Cat.-No AM20231PU). Species: Human.
-
纯化方法
- Protein G Chromatography
-
免疫原
- Purified Human small basement membrane Heparan Sulfate Proteoglycan (HS-PG)
-
亚型
- IgG1
-
-
-
-
应用备注
- ELISA (less than 1 μg/mL). Western blot (1 μg/mL). Immunohistochemistry (cryosections, 1 μg/mL).
-
实验流程
- 1. Stöcker G, Meyer HE, Wagener C and Greiling H (1991): Purification and N-terminal amino acid sequence of a chondroitin sulfate / dermatan sulfate proteoglycan isolated from intima / media preparations of human aorta. Biochem J 274: 415-420. 2. Heintz B, Stöcker G, Rentz U, Melzer H, Mrowka C, Stickeler E, Sieberth HG, Greiling H, Haubeck HD (1995): Decreased glomerular basement membrane heparan sulfate proteoglycan in essential hypertension. Hypertension 25: 399-407. 3. Stefanidis I, Heintz B, Stöcker G, Mrowka C, Sieberth HG, Haubeck HD (1996): Association between heparan sulfate proteoglycan excretion and proteinuria after renal transplantation. J Am Soc Nephrol 7: 1-7. 4. Stöcker G, Stickeler E, Switalla S, Fischer DC, Greiling H, Haubeck HD (1997): Development of an enzyme immuno assay specific for a core protein epitope of a novel small basement membrane associated heparan sulphate proteoglycan from human kidney. Eur J Clin Chem Clin Biochem 35: 95-99.
-
限制
- 仅限研究用
-
-
-
缓冲液
- 50 mM TRIS pH 7.4
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
-
有效期
- 12 months
-
-
- Small Basement Membrane Heparan Sulfate Proteoglycan
-
背景
- The principal molecular structure of basement membranes has been elucidated during the past two decades and it was shown that for example in the glomerular basement membrane (GBM) HS-PG are responsible for the selective filtration process. Removal of HS-PG will lead to proteinuria. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that (at least two) other basement membrane HS-PG do exist: Agrin, originally discovered as an important component of the neuromuscular junction and a novel small HS-PG, that was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200 kDa (aorta) and 30-160 kDa (kidney) and a core protein size of 24 kDa or 22 kDa, respectively, was localized by immunohisto-chemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.
-
UniProt
- P34741
抗原
-
You are here:
