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APP 抗体 (N-Term)

The 山羊 多克隆 anti-APP antibody is suitable to detect APP in samples from 人 和 小鼠. It has been validated for WB, ELISA 和 IHC.
产品编号 ABIN870659
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for APP 抗体 (N-Term) (ABIN870659)

抗原

See all APP 抗体
APP (Amyloid beta (A4) Precursor Protein (APP))

适用

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人, 小鼠

宿主

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山羊

克隆类型

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多克隆

标记

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This APP antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

质量等级

Verified
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    N-Term

    原理

    Amyloid beta peptide (mouse)

    序列

    DAEFGHDSGF EVRHQK

    特异性

    This antibody is expected to recognize all reported isoforms of mAPP (NP_001185752.1, NP_031497.2, NP_001185753.1, NP_001185754.1, NP_001185755.1) and to recognize all lengths of the putative mouse Amyloid beta peptide.

    交叉反应

    小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence DAEFGHDSGFEVRHQK-C, from the N Terminus of the protein sequence according to NP_001185752.1, NP_031497.2, NP_001185753.1, NP_001185754.1, NP_001185755.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5 μg/mL.

    Western Blot: Approx 100 kDa band observed in Mouse Brain lysates (calculated MW of 86.7 kDa according to NP_001185752.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    APP (Amyloid beta (A4) Precursor Protein (APP))

    别名

    App

    背景

    App, amyloid beta (A4) precursor protein, Abeta, Abpp, Adap, Ag, Cvap, E030013M08Rik, betaApp, alzheimer disease amyloid A4 protein homolog, amyloid beta A4 protein, amyloidogenic glycoprotein, appican, protease nexin II

    分子量

    86.7kDa

    基因ID

    11820, 54226

    NCBI登录号

    NP_001185752, NP_031497, NP_001185753, NP_001185754, NP_001185755

    途径

    Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour
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