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GRP78 抗体 (N-Term)

Name: GRP78 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN863190
Availability: OutOfStock

This anti-GRP78 antibody is a 兔多克隆 antibody detecting GRP78 in WB, ELISA, IHC, IF 和 ICC. Suitable for 人. This Primary Antibody has been cited in 3+ publications.

Glia GD lysate Western Blotting 1 in 1000 copy. (GRP78 抗体 (N-Term))

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 (Bip) Polyclonal Antibody (ABIN863189 and ABIN863190). (GRP78 抗体 (N-Term))

ICC

2 images

(3 references)

产品编号 ABIN863190

发货至: 中国

Quick Overview for GRP78 抗体 (N-Term) (ABIN863190)

抗原

See all GRP78 (HSPA5) 抗体

GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

适用

人

宿主

兔

克隆类型

多克隆

标记

This GRP78 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Discover our top product HSPA5 Primary Antibody

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N-Term

特异性

Detects ~78 kDa.

交叉反应

犬, Drosophila melanogaster, Hamster, 人, 小鼠, 大鼠

纯化方法

Protein A Purified

免疫原

Full length human GRP78 (Bip) his tagged at the N terminus
anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 550-650) antibody
HSPA5 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (Internal Region) antibody
HSPA5 适用: 人, 大鼠, 小鼠 WB, IF, ICC, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 251-360) antibody
HSPA5 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IF (cc), FACS, IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 550-650) antibody
HSPA5 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (HRP)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal HRP

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (FITC)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal FITC

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (Biotin)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Biotin

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (PE)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal PE

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (APC)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal APC

应用备注
- WB (1:2000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
说明

0.5 μg/ml of ABIN863189 was sufficient for detection of Grp78 in 10 μg of rat tissue lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

-20°C
Griffiths, Del Bel, Wilk, Brill: "Cellular homeostasis in the Drosophila retina requires the lipid phosphatase Sac1." in: Molecular biology of the cell, Vol. 31, Issue 11, pp. 1183-1199, (2020) (PubMed).

Liu, Chowdhury, Fang, Liu, Srikant: "Attenuation of unfolded protein response and apoptosis by mReg2 induced GRP78 in mouse insulinoma cells." in: FEBS letters, Vol. 588, Issue 11, pp. 2016-24, (2014) (PubMed).

Coelho, Cairrão, Zeng, Pires, Coelho, Ron, Ryoo, Domingos: "Xbp1-independent Ire1 signaling is required for photoreceptor differentiation and rhabdomere morphogenesis in Drosophila." in: Cell reports, Vol. 5, Issue 3, pp. 791-801, (2013) (PubMed).
抗原

GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

别名

GRP78

背景

GRP78 is a ubiquitously expressed, 78- kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).

基因ID

3309

NCBI登录号

NP_005338

UniProt

P11021

途径

Thyroid Hormone Synthesis, ER-Nucleus Signaling