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HSF1 抗体 (pSer307)

This anti-HSF1 antibody is a 兔 多克隆 antibody detecting HSF1 in WB, ELISA, IHC (p), IF (cc), IF (p) 和 IHC (fro). Suitable for 小鼠.
产品编号 ABIN800973
发货至: 中国

Quick Overview for HSF1 抗体 (pSer307) (ABIN800973)

抗原

See all HSF1 抗体
HSF1 (Heat Shock Factor Protein 1 (HSF1))

适用

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小鼠

宿主

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克隆类型

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  • 60
多克隆

标记

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This HSF1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 抗原表位

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    pSer307

    交叉反应

    小鼠

    预测反应

    Human

    纯化方法

    Purified by Protein A.

    免疫原

    KLH conjugated synthetic phosphopeptide derived from human HSF1 around the phosphorylation site of Ser303

    亚型

    IgG
  • 应用备注

    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 μg/μL

    缓冲液

    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    有效期

    12 months
  • 抗原

    HSF1 (Heat Shock Factor Protein 1 (HSF1))

    别名

    HSF1

    背景

    Synonyms: HSF1 Ser307, Heat shock factor 1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1, hsf1, HSTF 1, HSTF1, HSF1_HUMAN.

    Background: Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38 % sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells.HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.

    基因ID

    3297
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