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HSF1 抗体 (AA 378-395) (HRP)

HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 HRP
产品编号 ABIN2484629
发货至: 中国
  • 抗原 See all HSF1 抗体
    HSF1 (Heat Shock Factor Protein 1 (HSF1))
    抗原表位
    • 29
    • 15
    • 12
    • 12
    • 12
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 378-395
    适用
    • 193
    • 108
    • 57
    • 13
    • 11
    • 10
    • 7
    • 7
    • 6
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    小鼠
    宿主
    • 179
    • 32
    • 7
    • 1
    大鼠
    克隆类型
    • 168
    • 50
    单克隆
    标记
    • 118
    • 12
    • 12
    • 9
    • 8
    • 8
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This HSF1 antibody is conjugated to HRP
    应用范围
    • 182
    • 110
    • 84
    • 65
    • 47
    • 43
    • 30
    • 28
    • 28
    • 27
    • 9
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Gel Shift (GS), Immunocytochemistry (ICC)
    特异性
    Detects ~85 kDa (unstressed cell lysates), and~95 kDa (heat shocked cell lysates).
    交叉反应
    Cow, 豚鼠, Hamster, 人, 猴, 小鼠, 兔, 大鼠
    纯化方法
    Protein G Purified
    免疫原
    Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395
    克隆位点
    10H8
    亚型
    IgG1
    Top Product
    Discover our top product HSF1 Primary Antibody
  • 应用备注
    • WB (1:1000)
    • IHC (1:1000)
    • ICC/IF (1:200)
    • optimal dilutions for assays should be determined by the user.
    说明

    1 μg/ml of ABIN2484629 was sufficient for detection of HSF1 in 20 μg of heat shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Conjugated antibodies should be stored at 4°C
  • 抗原
    HSF1 (Heat Shock Factor Protein 1 (HSF1))
    别名
    HSF1 (HSF1 产品)
    别名
    hsf1 antibody, Hsf antibody, xhsf1 antibody, AA960185 antibody, HSTF1 antibody, HSF1 antibody, HSF antibody, HSF1c antibody, hsf1a antibody, hsf1b antibody, zHSF1a antibody, zHSF1b antibody, hstf1 antibody, heat shock factor antibody, Heat Shock Factor antibody, heat shock factor 1 antibody, heat shock transcription factor 1 antibody, heat shock transcription factor 1 S homeolog antibody, hsf1 antibody, hsf-1 antibody, Hsf1 antibody, HSF1 antibody, hsf1.S antibody
    背景
    HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
    基因ID
    15499
    NCBI登录号
    NP_032322
    UniProt
    P38532
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