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PDGFRA 抗体 (AA 1035-1053)

Cited in 1 publication. This 兔 多克隆 anti-PDGFRA antibody specifically detects PDGFRA in WB, IHC, IHC (p) 和 IP. The antibody is reactive with 人, 小鼠, 大鼠, 犬, Cow, 小鸡, 猴, Pig, 兔, 马, 非洲爪蟾, Bat 和 Hamster samples.
产品编号 ABIN790499
发货至: 中国
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Quick Overview for PDGFRA 抗体 (AA 1035-1053) (ABIN790499)

抗原

See all PDGFRA 抗体
PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

适用

人, 小鼠, 大鼠, 犬, Cow, 小鸡, 猴, Pig, 兔, 马, 非洲爪蟾, Bat, Hamster

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克隆类型

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多克隆

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This PDGFRA antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
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    AA 1035-1053

    特异性

    Recognizes human PDGF Receptor Type A, doublet, Mr 150/170kD. Species Crossreactivity: mouse. Species Sequence Homology: frog, chicken and zebra fish.

    预测反应

    Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%) Stickleback (95%) Zebrafish (89%).

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide corresponding to amino acids 1035-1053 (C-GKRNRHSSQTSEESAIETG) of human PDGF (KLH-conjugated). Receptor alpha. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%), Stickleback (95%), Zebrafish (89%).

    Type of Immunogen: Synthetic peptide - KLH conjugated

    亚型

    IgG
  • 应用备注

    Approved: IHC, IHC-P (1:100), IP, WB (1:250 - 1:1000)

    Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:250-1:1000 dilution detected PDGF Receptor a doublet (150/170kD) in 3T3/NIH cell lysate. 3T3/NIH cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PDGF Receptor a (1:500 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5-10 μL immunoprecipitates PDGF Receptor a from 500 μg of 3T3/NIH RIPA lysate.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.1 M Tris-glycine,  pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C

    储存方法

    May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use.
  • Nance, Shi, Hakim, Wasala, Yue, Pan, Zhang, Robinson, Duan, Yao, Yang, Chen, Wagner, Gersbach, Duan: "AAV9 Edits Muscle Stem Cells in Normal and Dystrophic Adult Mice." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 27, Issue 9, pp. 1568-1585, (2019) (PubMed).

  • 抗原

    PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

    别名

    PDGFRA / PDGFR Alpha

    背景

    Name/Gene ID: PDGFRA
    Subfamily: PDGF Receptor
    Family: Protein Kinase

    Synonyms: PDGFRA, CD140A, CD140a antigen, PDGFalphaR, PDGFR2, PDGFRalpha, AlphaPDGFR, RHEPDGFRA, PDGF receptor alpha, PDGFR-2, PDGFRA/BCR fusion, APDGFR, Pdgf alpha-receptor, PDGF-R-alpha, PDGFR-alpha

    基因ID

    5156

    UniProt

    P16234

    途径

    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling
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