PDGFRA 抗体 (AA 1035-1053)
Quick Overview for PDGFRA 抗体 (AA 1035-1053) (ABIN790499)
抗原
See all PDGFRA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1035-1053
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品牌
- IHC-plus™
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特异性
- Recognizes human PDGF Receptor Type A, doublet, Mr 150/170kD. Species Crossreactivity: mouse. Species Sequence Homology: frog, chicken and zebra fish.
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预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%) Stickleback (95%) Zebrafish (89%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide corresponding to amino acids 1035-1053 (C-GKRNRHSSQTSEESAIETG) of human PDGF (KLH-conjugated). Receptor alpha. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%), Stickleback (95%), Zebrafish (89%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
亚型
- IgG
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应用备注
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Approved: IHC, IHC-P (1:100), IP, WB (1:250 - 1:1000)
Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:250-1:1000 dilution detected PDGF Receptor a doublet (150/170kD) in 3T3/NIH cell lysate. 3T3/NIH cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PDGF Receptor a (1:500 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5-10 μL immunoprecipitates PDGF Receptor a from 500 μg of 3T3/NIH RIPA lysate. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C
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储存方法
- May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use.
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: "AAV9 Edits Muscle Stem Cells in Normal and Dystrophic Adult Mice." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 27, Issue 9, pp. 1568-1585, (2019) (PubMed).
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: "AAV9 Edits Muscle Stem Cells in Normal and Dystrophic Adult Mice." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 27, Issue 9, pp. 1568-1585, (2019) (PubMed).
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- PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))
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别名
- PDGFRA / PDGFR Alpha
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背景
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Name/Gene ID: PDGFRA
Subfamily: PDGF Receptor
Family: Protein Kinase
Synonyms: PDGFRA, CD140A, CD140a antigen, PDGFalphaR, PDGFR2, PDGFRalpha, AlphaPDGFR, RHEPDGFRA, PDGF receptor alpha, PDGFR-2, PDGFRA/BCR fusion, APDGFR, Pdgf alpha-receptor, PDGF-R-alpha, PDGFR-alpha -
基因ID
- 5156
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UniProt
- P16234
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling
抗原
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