The reactivity of the antiserum is restricted to lactoperoxidase as tested in immunoelectrophoresis and radial immunodiffusion. Using various antiserum concentrations against fresh bovine milk a single precipitin line is obtained. No precipitation reaction is obtained with normal bovine serum or plasma. Cross-reactivity: The antiserum does not cross-react with any other component of bovine serum. Interspecies cross-reactivity is a normal feature of antibodies, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, but reactivity with lactoperoxidase from goat and sheep may be expected.
纯化方法
DEAE-column Chromatography
免疫原
Lactoperoxidase (EC 1.11.1.7) is one of the major enzymes in bovine milk. The enzyme has a molecular weight of 80,000. It is isolated from pooled bovine milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Liquid
溶解方式
Restore by adding 1 mL sterile distilled water
浓度
10 mg/mL
缓冲液
PBS, pH 7.2 without preservatives and foreign proteins.
储存液
Without preservative
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.
抗原
LPO
(Lactoperoxidase (LPO))
别名
Lactoperoxidase
背景
Lactoperoxidase is a natural antibacterial agent in bovine milk, a medium otherwise rich in its ability to support bacterial growth. Milk lactoperoxidase levels vary among species, ranging from little or none in humans to very high concentrations in guinea pigs. Interestingly, lactoperoxidase from milk is identical to the lacrimal and salivary forms of the enzyme. In addition, the enzyme catalyzes the hydrogen peroxide oxidation of iodine and can be employed in labelling proteins with radioiodine.Synonyms: EC=1.11.1.7, LPO, Lactoperoxidase, SAPX, SPO, Salivary peroxidase